@article{PAR00009911,
  title = {{R}egulation of two forms of gonadotropin-releasing hormone receptor gene expression in the protandrous black porgy fish, {A}canthopagrus schlegeli},
  author = {{L}in, {C}. {J}. and {W}u, {G}. {C}. and {L}ee, {M}. {F}. and {L}au, {E}. {L}. and {D}ufour, {S}ylvie and {C}hang, {C}. {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}wo {G}n{RH} receptors ({G}n{RH}-{R} {I} and {G}n{RH}-{R} {II}) were obtained in protandrous black porgy ({A}canthopagrus schlegeli). {W}e investigated their tissue distribution, developmental/seasonal changes and regulation of expression using in vivo and in vitro (primary cultures of dispersed pituitary cells) approaches. {T}he relative expressions of {G}n{RH}-{R}s in the pituitary and gonad were as follows: pituitary: {G}n{RH}-{R} {I} > {G}n{RH}-{R} {II}; testicular tissue: {G}n{RH}-{R} {I} > {G}n{RH}-{R} {II}; ovarian tissue: {G}n{RH}-{R} {I} = {G}n{RH}-{R} {II}. {G}n{RH}-{R} {I} but not {G}n{RH}-{R} {II} expression was higher in the pituitary during the spawning period as compared to the prespawning. {T}he expression profiles of both forms of {G}n{RH}-{R} were variable in the gonads according to the gonadal stage and season. {I}n vivo, h{CG} stimualated {G}n{RH}-{R} {I} and {G}n{RH}-{R} {II} expression in testis and ovary. {T}he {LHRH} analog also up-regulated both receptors in testis and but increased only {G}n{RH}-{R} {II} in the ovary. {S}ex steroids (estradiol. {E}2 and testosterone, {T}) increased the expression of both receptors in the testis and ovary. {I}n the pituitary, sex steroids ({E}2 and {T}) increased the expression of {G}n{RH}-{R} {I}, but not {G}n{RH}-{II}, both in vivo and in vitro. {T}he {LHRH} analog also specifically up-regulated the expression of {G}n{RH}-{R} {I}, but not {G}n{RH}-{R} {II}, by pituitary cells in vitro. {A}ll these data suggest that {G}n{RH}-{R} {I} rather than {G}n{RH}-{R} {II} may play a major physiological role in the pituitary. {I}n contrast, both {G}n{RH}-{R} land {G}n{RH}-{R} {II} may participate in the regulation of gonadal functions, including a possible role during sex change.},
  keywords = {{F}ish ; {G}n{RH} ; {G}n{RH} receptor ; {S}ex steroids ; {P}ituitary cells},
  booktitle = {},
  journal = {{M}olecular and {C}ellular {E}ndocrinology},
  volume = {323},
  numero = {2},
  pages = {137--146},
  ISSN = {0303-7207},
  year = {2010},
  DOI = {10.1016/j.mce.2010.04.003},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00009911},
}