Kempf M. auteur aut IRD Rolain J. M. auteur aut IRD Diatta G. auteur aut IRD Azza S. auteur aut IRD Samb B. auteur aut IRD Mediannikov Oleg auteur aut IRD Sow A. G. auteur aut IRD Diene S. M. auteur aut IRD Fenollar F. auteur aut IRD Raoult D. auteur aut IRD text journalArticle eng text/pdf reformatted digital access Incidence of carbapenem-resistant Acinetobacter baumannii is rising in several parts of the world. In Africa, data concerning this species and its resistance to carbapenems are limited. The objective of the present study was to identify the presence of A. baumannii carbapenem-resistant encoding genes in natural reservoirs in Senegal, where antibiotic pressure is believed to be low. From October 2010 to January 2011, 354 human head lice, 717 human fecal samples and 118 animal fecal samples were screened for the presence of A. baumannii by real time PCR targeting bla(OXA51-like) gene. For all samples positive for A. baumannii, the carbapenemase-hydrolysing oxacillinases bla(OXA23-like) and bla(OXA24-like) were searched for and sequenced, and the isolates harbouring an oxacillinase were genotyped using PCR amplification and sequencing of recA gene. The presence of A. baumannii was detected in 4.0% of the head lice, in 5.4% of the human stool samples and in 5.1% of the animal stool samples tested. No bla(OXA24) gene was detected but six fecal samples and three lice were positive for bla(OXA23-like) gene. The bla(OXA23-like) gene isolated in lice was likely a new oxacillinase sequence. Finally, the A. baumannii detected in stools were all of recA genotype 3 and those detected in lice, of recA genotype 4. This study shows for the first time a reservoir of bla(OXA23-like)-positive gene in human head lice and stool samples in Senegal. specialized 052 7 6 e39495 2012 1932-6203 https://www.documentation.ird.fr/hor/PAR00009076 10.1371/journal.pone.0039495 1932-6203 [F B010055992] https://www.documentation.ird.fr/hor/PAR00009076 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-09/010055992.pdf IRD - Base Horizon / Pleins textes 2012-07-24 2025-06-13 PAR00009076 fre