@article{PAR00008216,
  title = {{F}lavivirus {NS}3 and {NS}5 proteins interaction network: a high-throughput yeast two-hybrid screen},
  author = {{L}e {B}reton, {M}. and {M}eyniel-{S}chicklin, {L}. and {D}eloire, {A}. and {C}outard, {B}. and {C}anard, {B}. and de {L}amballerie, {X}avier and {A}ndre, {P}. and {R}abourdin-{C}ombe, {C}. and {L}otteau, {V}. and {D}avoust, {N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}he genus {F}lavivirus encompasses more than 50 distinct species of arthropod-borne viruses, including several major human pathogens, such as {W}est {N}ile virus, yellow fever virus, {J}apanese encephalitis virus and the four serotypes of dengue viruses ({DENV} type 1-4). {E}ach year, flaviviruses cause more than 100 million infections worldwide, some of which lead to life-threatening conditions such as encephalitis or haemorrhagic fever. {A}mong the viral proteins, {NS}3 and {NS}5 proteins constitute the major enzymatic components of the viral replication complex and are essential to the flavivirus life cycle. {R}esults: {W}e report here the results of a high-throughput yeast two-hybrid screen to identify the interactions between human host proteins and the flavivirus {NS}3 and {NS}5 proteins. {U}sing our screen results and literature curation, we performed a global analysis of the {NS}3 and {NS}5 cellular targets based on functional annotation with the {G}ene {O}ntology features. {W}e finally created the first flavivirus {NS}3 and {NS}5 proteins interaction network and analysed the topological features of this network. {O}ur proteome mapping screen identified 108 human proteins interacting with {NS}3 or {NS}5 proteins or both. {T}he global analysis of the cellular targets revealed the enrichment of host proteins involved in {RNA} binding, transcription regulation, vesicular transport or innate immune response regulation. {C}onclusions: {W}e proposed that the selective disruption of these newly identified host/virus interactions could represent a novel and attractive therapeutic strategy in treating flavivirus infections. {O}ur virus-host interaction map provides a basis to unravel fundamental processes about flavivirus subversion of the host replication machinery and/or immune defence strategy.},
  keywords = {},
  booktitle = {},
  journal = {{B}mc {M}icrobiology},
  volume = {11},
  numero = {},
  pages = {234},
  ISSN = {1471-2180},
  year = {2011},
  DOI = {10.1186/1471-2180-11-234},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00008216},
}