@article{PAR00008215,
  title = {{C}omparative detection of enterovirus {RNA} in cerebrospinal fluid : {G}ene{X}pert system vs. real-time {RT}-{PCR} assay},
  author = {{N}inove, {L}. and {N}ougairede, {A}. and {G}azin, {C}. and {Z}andotti, {C}. and {D}rancourt, {M}. and de {L}amballerie, {X}avier and {C}harrel, {R}.},
  editor = {},
  language = {{ENG}},
  abstract = {{E}nteroviruses ({EV}s) constitute the most common cause of aseptic meningitis in both children and adults. {M}olecular techniques have now been recognized as the reference standard for the diagnosis of {EV} infections, and the rapidity of the molecular diagnosis of {EV} meningitis has been shown to be a determining factor in the management of patients. {T}he rapid documentation of {EV} {RNA} in cerebrospinal fluid ({CSF}) is key to adapting patient management and the therapeutic regimen. {T}o shorten the time needed for virological documentation, we implemented {EV} {RNA} detection in two point-of-care ({POC}) laboratories. {H}ere, we present the results of the {POC} detection of {EV} {RNA} with the {X}pert {EV} kit on the {G}ene{X}pert integrated system, and a comparison with the real-time {RT}-{PCR} (rt{RT}-{PCR}) assay routinely used in the core virology laboratory. {F}rom {J}anuary to {S}eptember 2009, a total of 310 {CSF} samples were tested. {T}he rt{RT}-{PCR} gave 81 positive, 225 negative and four indeterminate results. {POC} results were concordant in 81.6% (253/310). {M}ost of the discrepancies consisted of indeterminate results at the {POC} level (16%). {C}alculated performances (excluding the indeterminate results) of the {X}pert {EV} kit on the {G}ene{X}pert system in {POC} settings were 100%, 98.9%, 97.6% and 100% for {S}ensibility, {S}pecificity, positive predictive value and negative predictive value, respectively. {T}aken together, these results indicate that the implementation of {POC} detection of {EV} {RNA} can provide robust results in <4 h, and may have a significant impact on patient management, therapeutic attitude, and hospitalization costs.},
  keywords = {{C}erebrospinal fluid ; enterovirus ; point-of-care ; rapid diagnosis ; real-time {PCR}},
  booktitle = {},
  journal = {{C}linical {M}icrobiology and {I}nfection},
  volume = {17},
  numero = {12},
  pages = {1890--1894},
  ISSN = {1198-743{X}},
  year = {2011},
  DOI = {10.1111/j.1469-0691.2011.03487.x},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00008215},
}