Ruiz C. auteur aut IRD Haddad Mohamed auteur aut IRD Alban J. auteur aut IRD Bourdy Geneviève auteur aut IRD Reategui R. auteur aut IRD Castillo D. auteur aut IRD Sauvain Michel auteur aut IRD Deharo Eric auteur aut IRD Estevez Yannick auteur aut IRD Arevalo J. auteur aut IRD Rojas R. auteur aut IRD text journalArticle eng text/pdf born digital access The bioassay-guided purification of the ethanolic extract from the leaves of Piper hispidum led to the isolation of one new amide, N-2-(3',4',5'-trimethoxyphenyl)ethyl-2-hydroxybenzamide (1) as well as two known chalcones 2'-hydroxy-3',4',6'-trimethoxychalcone (2); 2',4'-dihydroxy-6'-methoxychalcone (cardamonin, 3) and one known flavanone, 5,7-dihydroxyflavanone (Pinocembrin, 4). Their structures were elucidated on the basis of spectroscopic data, including homo-and heteronuclear correlation NMR experiments (COSY, HSQC and HMBC) and comparison with data reported in the literature. The isolated compounds were tested against Leishmania amazonensis axenic amastigotes. The results showed that the known chalcone 2 exhibited the most potent antileishmanial activity with an IC50 of 0.8 mu M (amphotericin B: IC50 = 0.2 mu M) but was shown to exhibit mild cytotoxicity. specialized Piper Amide Chalcone Flavanone Leishmaniasis 076 052 4 3 363-366 2011 1874-3900 https://www.documentation.ird.fr/hor/PAR00008110 10.1016/j.phytol.2011.08.001 1874-3900 [F B010054162] https://www.documentation.ird.fr/hor/PAR00008110 https://www.documentation.ird.fr/intranet/publi/2011/11/010054162.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2011-11-30 2017-08-23 PAR00008110 fre