@article{PAR00007899,
  title = {{T}he {NTS}-{DBL}2{X} region of {VAR}2{CSA} induces cross-reactive antibodies that inhibit adhesion of several {P}lasmodium falciparum {I}solates to {C}hondroitin {S}ulfate {A}},
  author = {{B}igey, {P}. and {G}nidehou, {S}{\'e}dami and {J}ustin, {D}. and {Q}uiviger, {M}. and {V}iwami, {F}. and {C}outurier, {A}. and {S}alanti, {A}. and {N}ielsen, {M}. {A}. and {S}cherman, {D}. and {D}eloron, {P}hilippe and {T}uikue {N}dam, {N}icaise},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground. {B}inding to chondroitin sulfate {A} by {VAR}2{CSA}, a parasite protein expressed on infected erythrocytes, allows placental sequestration of {P}lasmodium falciparum-infected erythrocytes. {T}his leads to severe consequences such as maternal anemia, stillbirths, and intrauterine growth retardation. {T}he latter has been clearly associated to increased morbidity and mortality of the infants. {A}cquired anti-{VAR}2{CSA} antibodies have been associated with improved pregnancy outcomes, suggesting a vaccine could prevent the syndrome. {H}owever, identifying functionally important regions in the large {VAR}2{CSA} protein is difficult. {M}ethods. {U}sing genetic immunization, we raised polyclonal antisera against overlapping segments of {VAR}2{CSA} in mice and rabbits. {T}he adhesion-inhibition capacities of induced antisera and of specific antibodies purified from plasma of malaria-exposed pregnant women were assessed on laboratory-adapted parasite lines and field isolates expressing {VAR}2{CSA}. {C}ompetition enzyme-linked immunosorbent assay ({ELISA}) was employed to analyze functional resemblance between antibodies induced in animals and those naturally acquired by immune multigravidae. {R}esults. {A}ntibodies targeting the {N}-terminal sequence ({NTS}) up to {DBL}2{X} ({NTS}-{DBL}2{X}) efficiently blocked parasite adhesion to chondroitin sulfate {A} in a manner similar to that of antibodies raised against the entire {VAR}2{CSA} extracellular domain. {I}nterestingly, naturally acquired antibodies and those induced by vaccination against {NTS}-{DBL}2{X} target overlapping strain-transcendent anti-adhesion epitopes. {C}onclusions. {T}his study highlights an important step achieved toward development of a protective vaccine against placental malaria.},
  keywords = {},
  booktitle = {},
  journal = {{J}ournal of {I}nfectious {D}iseases},
  volume = {204},
  numero = {7},
  pages = {1125--1133},
  ISSN = {0022-1899},
  year = {2011},
  DOI = {10.1093/infdis/jir499},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00007899},
}