Autran Daphné auteur aut IRD Baroux C. auteur aut IRD Raissig M.T. auteur aut IRD Lenormand T. auteur aut IRD Wittig M. auteur aut IRD Grob S. auteur aut IRD Steimer A. auteur aut IRD Barann M. auteur aut IRD Klostermeier U.C. auteur aut IRD Leblanc Olivier auteur aut IRD Vielle-Calzada J.P. auteur aut IRD Rosenstiel P. auteur aut IRD Grimanelli Daniel auteur aut IRD Grossniklaus U. auteur aut IRD text journalArticle eng Defining the contributions and interactions of paternal and maternal genomes during embryo development is critical to understand the fundamental processes involved in hybrid vigor, hybrid sterility, and reproductive isolation. To determine the parental contributions and their regulation during Arabidopsis embryogenesis, we combined deep-sequencing-based RNA profiling and genetic analyses. At the 2-4 cell stage there is a strong, genome-wide dominance of maternal transcripts, although transcripts are contributed by both parental genomes. At the globular stage the relative paternal contribution is higher, largely due to a gradual activation of the paternal genome. We identified two antagonistic maternal pathways that control these parental contributions. Paternal alleles are initially downregulated by the chromatin siRNA pathway, linked to DNA and histone methylation, whereas transcriptional activation requires maternal activity of the histone chaperone complex CAF1. Our results define maternal epigenetic pathways controlling the parental contributions in plant embryos, which are distinct from those regulating genomic imprinting. specialized 076 145 5 707-719 2011 0092-8674 https://www.documentation.ird.fr/hor/PAR00007635 10.1016/j.cell.2011.04.014 0092-8674 https://www.documentation.ird.fr/hor/PAR00007635 IRD - Base Horizon / Pleins textes 2011-07-01 2017-08-23 PAR00007635 fre