@article{PAR00006853,
  title = {{U}se of the atmospheric generators for capnophilic bacteria {G}enbag-{CO}2 for the evaluation of in vitro {P}lasmodium falciparum susceptibility to standard anti-malarial drugs},
  author = {{P}ascual, {A}. and {B}asco, {L}eonardo and {B}aret, {E}. and {A}malvict, {R}. and {T}ravers, {D}. and {R}ogier, {C}. and {P}radines, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}he aim of this study was to evaluate the cultivation system in which the proper atmospheric conditions for growing {P}lasmodium falciparum parasites were maintained in a sealed bag. {T}he {G}enbag ({R}) system associated with the atmospheric generators for capnophilic bacteria {G}enbag {CO}2 ({R}) was used for in vitro susceptibility test of nine standard anti-malarial drugs and compared to standard incubator conditions. {M}ethods: {T}he susceptibility of 36 pre-identified parasite strains from a wide panel of countries was assessed for nine standard anti-malarial drugs (chloroquine, quinine, mefloquine, monodesethylamodiaquine, lumefantrine, dihydroartemisinin, atovaquone and pyrimethamine) by the standard 42-hour {H}-3-hypoxanthine uptake inhibition method using the {G}enbag {CO}2 ({R}) system and compared to controlled incubator conditions (5% {CO}2 and 10% {O}-2). {R}esults: {T}he counts per minute values in the control wells in incubator atmospheric conditions (5% {CO}2 and 10% {O}-2) were significantly higher than those of {G}enbag ({R}) conditions (2738 cpm vs 2282 cpm, p < 0.0001). {T}he geometric mean {IC}50 estimated under the incubator atmospheric conditions was significantly lower for atovaquone (1.2 vs 2.1 n{M}, p = 0.0011) and higher for the quinolines: chloroquine (127 vs 94 n{M}, p < 0.0001), quinine (580 vs 439 n{M}, p < 0.0001), monodesethylamodiaquine (41.4 vs 31.8 n{M}, p < 0.0001), mefloquine (57.5 vs 49.7 n{M}, p = 0.0011) and lumefantrine (23.8 vs 21.2 n{M}, p = 0.0044). {T}here was no significant difference of {IC}50 between the 2 conditions for dihydroartemisinin, doxycycline and pyrimethamine. {T}o reduce this difference in term of anti-malarial susceptibility, a specific cut-off was estimated for each drug under {G}enbag ({R}) conditions by regression. {T}he cut-off was estimated at 77 n{M} for chloroquine (vs 100 n{M} in 10% {O}-2), 611 n{M} for quinine (vs 800 n{M}), 30 n{M} for mefloquine (vs 30 n{M}), 61 n{M} for monodesethylamodiaquine (vs 80 n{M}) and 1729 n{M} for pyrimethamine (vs 2000 n{M}). {C}onclusions: {T}he atmospheric generators for capnophilic bacteria {G}enbag {CO}2 ({R}) is an appropriate technology that can be transferred to the field for epidemiological surveys of drug-resistant malaria. {T}he present data suggest the importance of the gas mixture on in vitro microtest results for anti-malarial drugs and the importance of determining the microtest conditions before comparing and analysing the data from different laboratories and concluding on malaria resistance.},
  keywords = {{SENEGAL}},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {10},
  numero = {},
  pages = {8},
  ISSN = {1475-2875},
  year = {2011},
  DOI = {10.1186/1475-2875-10-8},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00006853},
}