@article{PAR00006581,
  title = {{A} derivate of the antibiotic doxorubicin is a selective inhibitor of dengue and yellow fever virus replication in vitro},
  author = {{K}aptein, {S}. {J}. {F}. and {D}e {B}urghgraeve, {T}. and {F}roeyen, {M}. and {P}astorino, {B}. and {A}len, {M}. {M}. {F}. and {M}ondotte, {J}. {A}. and {H}erdewijn, {P}. and {J}acobs, {M}. and de {L}amballerie, {X}avier and {S}chols, {D}. and {G}amarnik, {A}. {V}. and {S}ztaricskai, {F}. and {N}eyts, {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A} doxorubicin derivate, {SA}-17, that carries a squaric acid amide ester moiety at the carbohydrate (alpha-{L}-daunosaminyl) group was identified as a selective inhibitor of in vitro dengue virus ({DENV}) serotype 2 replication (50% effective concentration [{EC}50] = 0.34 +/- 0.20 mu g/ml [0.52 +/- 0.31 mu {M}]). {SA}-17 is markedly less cytostatic than the parent compound, resulting in a selectivity index value of similar to 100. {SA}-17 also inhibits yellow fever virus 17{D} ({YFV}-17{D}) replication ({EC}50 = 3.1 +/- 1.0 mu g/ml [4.8 +/- 1.5 mu {M}]), although less efficiently than {DENV} replication, but proved inactive against a variety of enveloped and nonenveloped viruses. {SA}-17 inhibits in vitro flavivirus replication in a dose-dependent manner, as was assessed by virus yield reduction assays and quantification of viral {RNA} by means of real-time quantitative reverse transcriptase {PCR} ({RT}-q{PCR}) (similar to 2 to 3 log reduction). {T}he anti-{DENV} activity was confirmed using a {R}enilla luciferase-expressing dengue reporter virus. {T}ime-of-drug-addition studies revealed that {SA}-17 acts at the very early stages of the viral replication cycle (i.e., virus attachment and/or virus entry). {T}his observation was corroborated by the observation that {SA}-17, unlike the nucleoside analogue ribavirin, does not inhibit the replication of {DENV} subgenomic replicons. {P}reincubation of high-titer stocks of {DENV} or {YFV}-17{D} with >= 5 mu g/ml {SA}-17 resulted in 100% inhibition of viral infectivity (>= 3 log reduction). {SA}-17, however, did not prove virucidal.},
  keywords = {},
  booktitle = {},
  journal = {{A}ntimicrobial {A}gents and {C}hemotherapy},
  volume = {54},
  numero = {12},
  pages = {5269--5280},
  ISSN = {0066-4804},
  year = {2010},
  DOI = {10.1128/aac.00686-10},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00006581},
}