@article{PAR00006562,
  title = {{S}omatic stem cells express {P}iwi and {V}asa genes in an adult ctenophore : ancient association of "germline genes" with stemness},
  author = {{A}lie, {A}. and {L}eclere, {L}. and {J}ager, {M}. and {D}ayraud, {C}. and {C}hang, {P}. {R}. and {L}e {G}uyader, {H}erv{\'e} and {Q}ueinnec, {E}. and {M}anuel, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}tem cells are essential for animal development and adult tissue homeostasis, and the quest for an ancestral gene fingerprint of sternness is a major challenge for evolutionary developmental biology. {R}ecent studies have indicated that a series of genes, including the transposon silencer {P}iwi and the translational activator {V}asa, specifically involved in germline determination and maintenance in classical bilaterian models (e.g., vertebrates, fly, nematode), are more generally expressed in adult multipotent stem cells in other animals like flatworms and hydras. {S}ince the progeny of these multipotent stem cells includes both somatic and germinal derivatives, it remains unclear whether {V}asa, {P}iwi, and associated genes like {B}runo and {PL}10 were ancestrally linked to sternness, or to germinal potential. {W}e have investigated the expression of {V}asa, two {P}iwi paralogues, {B}runo and {PL}10 in {P}leurobrachia pileus, a member of the early-diverging phylum {C}tenophora, the probable sister group of cnidarians. {T}hese genes were all expressed in the male and female germlines, and with the exception of one of the {P}iwi paralogues, they showed similar expression patterns within somatic territories (tentacle root, comb rows, aboral sensory complex). {C}ytological observations and {E}d{U} {DNA}-labelling and long-term retention experiments revealed concentrations of stem cells closely matching these gene expression areas. {T}hese stem cell pools are spatially restricted, and each specialised in the production of particular types of somatic cells. {T}hese data unveil important aspects of cell renewal within the ctenophore body and suggest that {P}iwi, {V}asa, {B}runo, and {PL}10 belong to a gene network ancestrally acting in two distinct contexts: (i) the germline and (ii) stem cells, whatever the nature of their progeny.},
  keywords = {{C}tenophore ; {E}volution ; {G}ermline ; {P}iwi ; {S}tem cell ; {V}asa},
  booktitle = {},
  journal = {{D}evelopmental {B}iology},
  volume = {350},
  numero = {1},
  pages = {183--197},
  ISSN = {0012-1606},
  year = {2011},
  DOI = {10.1016/j.ydbio.2010.10.019},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00006562},
}