@article{PAR00006015,
  title = {{E}ffect of storage conditions of dried plasma and blood spots on {HIV}-1 {RNA} quantification and {PCR} amplification for drug resistance genotyping},
  author = {{M}onleau, {M}. and {B}utel, {C}hristelle and {D}elaporte, {E}ric and {B}oillot, {F}. and {P}eeters, {M}artine},
  editor = {},
  language = {{ENG}},
  abstract = {{D}ried blood spots ({DBS}) and dried plasma spots ({DPS}) are easy to collect and store, and have been successfully tested as an alternative to plasma for performing virological analyses. {A}dequate storage conditions still need to be established and cell-associated proviral {DNA} in {DBS} can contribute to the amplified products. {W}e evaluated these two parameters. {R}esidual samples from 34 {HIV}-1-infected patients [mean viral load ({VL}) = 3.93 log(10) copies/m{L}] were used to prepare {DPS} and {DBS}, then stored at 20 degrees {C} and 37 degrees {C}. {HIV}-1 nucleic acids were extracted, with or without {DN}ase treatments, to perform {HIV}-1 {VL} quantification and nested {RT}-{PCR} to amplify the reverse transcriptase gene (798 bp). {F}or {DBS} stored for 3 months at 20 degrees {C}, {VL} could be measured for all samples and results were comparable to plasma {VL}. {A}t 37 degrees {C}, a slight decrease was observed after 2 and 3 months (0.16 and 0.37 log(10) copies/m{L} mean difference, respectively). {F}or {DPS}, a significant decrease in {VL} (0.70 and 1.07 log(10) copies/m{L} after 1 and 2 months, respectively) was seen at 37 degrees {C}, but not at 20 degrees {C}. {PCR} amplifications from {DPS} were only successful for 50% of samples with an initial {VL} > 10 000 copies/m{L} after 1 month at 20 degrees {C}. {F}rom {DBS}, {PCR} amplifications are possible until 3 months for samples with plasma {VL} > 5000 copies/m{L}. {VL} and {PCR} results for {DBS} treated with {DN}ase are close to results obtained for {DPS}. {V}irological monitoring is still feasible for {DBS} after 3 months of storage at 37 degrees {C} when {VL} is > 5000 copies/m{L}, but {DNA} contributes largely to the final results.},
  keywords = {903 filter paper ; drug resistance surveillance ; viral load ; {RNA} stability ; dried spot conservation ; resource-limited countries ; {DBS} ; {DPS}},
  booktitle = {},
  journal = {{J}ournal of {A}ntimicrobial {C}hemotherapy},
  volume = {65},
  numero = {8},
  pages = {1562--1566},
  ISSN = {0305-7453},
  year = {2010},
  DOI = {10.1093/jac/dkq205},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00006015},
}