@article{PAR00005767,
  title = {{A} simple method for molecular detection of swine-origin and human-origin influenza {A} virus},
  author = {{N}inove, {L}. and {G}azin, {C}. and {G}ould, {E}. {A}. and {N}ougairede, {A}. and {F}lahault, {A}. and {C}harrel, {R}. {N}. and {Z}andotti, {C}. and de {L}amballerie, {X}avier},
  editor = {},
  language = {{ENG}},
  abstract = {{H}ere we present a real-time one-step reverse transcriptase-polymerase chain reaction {SYBR} {G}reen assay derived from the method reported by van {E}lden and collaborators (2001) that ensures the rapid, sensitive, and cost-effective detection of both seasonal influenza {A} virus and emerging ({H}1{N}1) swine-origin influenza {A} virus ({S}-{OIV}). {I}n addition to this screening test, which successfully detected both seasonal influenza {A} virus and {S}-{OIV} in human clinical samples, we showed that the probe initially designed by van {E}lden and collaborators could detect seasonal influenza {A} virus, but not {S}-{OIV}; a new probe was designed and tested that specifically detects {S}-{OIV}, but not seasonal influenza {A}. {B}oth probe-based assays were validated by testing human clinical samples and specifically detected either seasonal influenza virus or {S}-{OIV}. {F}inally, in silico analysis of databases predicted that minor modifications of the van {E}lden primers would facilitate the use of this assay for the broad spectrum detection of all currently characterized variants of influenza {A} virus, including avian strains.},
  keywords = {{A}rbovirus(es) ; {C}hikungunya ; {D}engue virus ; {T}ick-borne encephalitis ; {V}ector-borne},
  booktitle = {},
  journal = {{V}ector-{B}orne and {Z}oonotic {D}iseases},
  volume = {10},
  numero = {3},
  pages = {237--240},
  ISSN = {1530-3667},
  year = {2010},
  DOI = {10.1089/vbz.2009.0110},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00005767},
}