@article{PAR00004533,
  title = {{D}evelopment of alternative plant vitrification solutions in droplet-vitrification procedures},
  author = {{K}im, {H}. {H}. and {L}ee, {Y}. {G}. and {S}hin, {D}. {J}. and {K}o, {H}. {C}. and {G}wag, {J}. {G}. and {C}ho, {E}. {G}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{T}his study aimed at developing alternative vitrification solutions, modified either from the original {PVS}2 vitrification solution, by increasing glycerol and sucrose and/or decreasing dimethylsulfoxide and ethylene glycol concentration, or from the original {PVS}3 vitrification solution by decreasing glycerol and sucrose concentration. {T}he application of these vitrification solutions to two model species, i.e. garlic and chrysanthemum in a droplet-vitrification procedure, revealed that {PVS}3 and variants were superior to {PVS}2 and variants and that most {PVS}2 variants were comparable to the original {PVS}2. {B}oth species were sensitive to chemical toxicity of permeating cryoprotectants and chrysanthemum was also sensitive to osmotic stress. {T}he lower recovery of cryopreserved garlic shoot apices dehydrated with {PVS}2 and variants compared with those dehydrated with {PVS}3 and variants seemed attributed to cytotoxicity of the vitrification solutions tested as well as to insufficient protection against freezing injury. {C}hrysanthemum shoot tips were very sensitive to both chemical toxicity and osmotic stress and therefore, induction of cytotoxity tolerance during preconditioning was required for successful cryopreservation. {T}he present study revealed that some of the {PVS}2 variants tested which have increased glycerol and sucrose and/or decreased dimethylsulfoxide and ethylene glycol concentration can be applied when explants are of medium size, tolerant to chemical toxicity and moderately sensitive to osmotic stress. {PVS}3 and variants can be used widely when samples are heterogeneous, of large size and/or very sensitive to chemical toxicity and tolerant to osmotic stress.},
  keywords = {chrysanthemum ; dehydration ; droplet-vitrification ; garlic ; vitrification solution},
  booktitle = {},
  journal = {{C}ryoletters},
  volume = {30},
  numero = {5},
  pages = {320--334},
  ISSN = {0143-2044},
  year = {2009},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00004533},
}