Klouche N. auteur aut IRD Basso O. auteur aut IRD Lascourreges J. F. auteur aut IRD Cayol J-L. auteur aut IRD Thomas P. auteur aut IRD Fauque G. auteur aut IRD Fardeau Marie-Laure auteur aut IRD Magot M. auteur aut IRD text journalArticle eng text/pdf reformatted digital access A novel anaerobic, chemo-organotrophic bacterium, designated VNs36(T), was isolated from a well that collected water from a deep saline aquifer used for underground gas storage at a depth of 830 m in the Paris Basin, France. Cells were curved motile rods or vibrios (3.0-5.0x0.5 mu m). Strain VNs36(T) grew at temperatures between 20 and 50 degrees C (optimum 37 degrees C) and at pH values between 5.0 and 9.0 (optimum 6.9). It did not require salt for growth, but tolerated up to 20 g NaCl l(-1) (optimum 2 g l(-1)). In the presence of sulfate, strain VNs36(T) used lactate, formate and pyruvate as carbon and energy sources. The main fermentation products from lactate were acetate, H-2 and CO2. Sulfate, thiosulfate and sulfite were used as electron acceptors, but not sulfur. The genomic DNA G+C content of strain VNs36(T) was 67.2 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain VNs36(T) was affiliated with the family Desulfovibrionaceae within the class Deltaproteobacteria. On the basis of 16S rRNA gene sequence comparisons, DNA G + C content and the absence of desulfoviridin in cell extracts, it is proposed that strain VNs36(T) be assigned to a new genus, Desulfocurvus gen. nov., as a representative of a novel species, Desulfocurvus vexinensis sp. nov. The type species of this genus is Desulfocurvus vexinensis with the type strain VNs36(T) (= DSM 17965(T) = JCM 14038(T)). specialized 084 064 59 Part 12 3100-3104 2009 1466-5026 https://www.documentation.ird.fr/hor/PAR00004444 10.1099/ijs.0.010363-0 1466-5026 [F B010091838] https://www.documentation.ird.fr/hor/PAR00004444 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2025-02/010091838.pdf IRD - Base Horizon / Pleins textes 2010-02-08 2025-02-19 PAR00004444 fre