@article{PAR00004441, title = {{DNA} methylation in {A}cacia mangium in vitro and ex-vitro buds, in relation to their within-shoot position, age and leaf morphology of the shoots}, author = {{M}onteuuis, {O}. and {B}aurens, {F}. {C}. and {G}oh, {D}. {K}. {S}. and {Q}uimado, {M}. and {D}oulbeau, {S}ylvie and {V}erdeil, {J}. {L}.}, editor = {}, language = {{ENG}}, abstract = {{DNA} methylation was analyzed in {A}cacia mangium {W}illd. buds collected from in vitro and field growing shoots from the same clone and its juvenile offspring. {S}hoot tips from in vitro microshoots showed higher levels of {DNA} methylation for juvenile (23.4%) than for mature (21.8%) plant material, irrespective of their morphology. {L}ikewise, under the same in vitro conditions, the apical buds collected from microshoots exhibiting the juvenile morphology displayed higher {DNA} methylation rates (23.2%) than those characterized by the mature phyllode morphological type (22.1%), regardless of the age of the plant material source. {I}n outdoor conditions, apical buds collected from mature tree growing shoots were significantly less methylated (20.9%) than the resting axillary buds located just underneath (25.2%) and than the apical buds from the in vitro microshoots of the same clone and mature-like phyllode morphology (22.6%). {O}verall, the {DNA} methylation levels found for our {A}cacia mangium plant materials were consistent with those reported for other {A}ngiosperms, but showed, similarly to other recent findings in different species, that {DNA} methylation in plants does not always increase with ageing. {T}his was discussed in relation to other phase-related traits and more particularly foliage characteristics, which are conspicuous markers of ageing in this heteroblastic species.}, keywords = {{A}cacia mangium ; {A}geing ; {DNA} methylation ; {I}n vitro ; {M}orphological ; markers ; {W}ithin-shoot position}, booktitle = {}, journal = {{S}ilvae {G}enetica}, volume = {58}, numero = {5-6}, pages = {287--292}, ISSN = {0037-5349}, year = {2009}, URL = {https://www.documentation.ird.fr/hor/{PAR}00004441}, }