@article{PAR00004142, title = {{M}olecular characterisation of the sucrose transporter {H}b{SUT}1{B} in relation to rubber production in latex cells}, author = {{D}usotoit-{C}oucaud, {A}. and {B}runel, {N}. and {F}ranchel, {J}. and {G}ranet, {F}. and {C}hrestin, {H}erv{\'e} and {S}akr, {S}.}, editor = {}, language = {{ENG}}, abstract = {{T}he rubber synthesis in latex cells requires sucrose. {T}hese particular cells are heterotrophic ones and are apoplastically connected to other tissues. {S}uch features imply that the rubber yield may depend on the latex cell efficiency to absorb and to use the imported sucrose. {T}his study investigates the sucrose transporters as key genes for rubber biosynthesis involved in the ethylene-stimulated rubber yield. {S}even putative sucrose transporters had been isolated from a latex-derived c{DNA} library of {PB} 217. {E}xpression profiling of these sucrose transporters under ethylene stimulation in {PB} 217 underlined the role of one sucrose transporter: {H}b{SUT}1{B} ({H}evea brasiliensis {S}ucrose {T}ransporter 6) ({D}usotoit-{C}oucaud et al., 2007). {T}his paper reports the next stage in {H}b{SUT}1{B}, molecular characterisation. {I}ts expression in different parts of the rubber tree was studied. {I}t was preferentially expressed in leaves and bark. {T}his result was completed by in-situ hybridisation experiments. {I}n addition, promoter cloning and analysis revealed some clues for the wounding regulation of {H}b{SUT}1{B}. {T}he {H}b{SUT}1{B} expression was analysed in response to different treatments in latex or bark tissues. {H}b{SUT}1{B} was considerably up-regulated by hormones and wounding in bark but not in latex. {T}his regulation appeared to be tissue-specific.}, keywords = {sucrose transporters ; {H}b{SUT}1{B} ; rubber production ; latex cells ; molecular regulation}, booktitle = {}, journal = {{J}ournal of {R}ubber {R}esearch}, volume = {12}, numero = {3}, pages = {125--139}, ISSN = {1511-1768}, year = {2009}, URL = {https://www.documentation.ird.fr/hor/{PAR}00004142}, }