Cachet N. auteur aut IRD Hoakwie F. auteur aut IRD Bertani S. auteur aut IRD Bourdy Geneviève auteur aut IRD Deharo Eric auteur aut IRD Stien D. auteur aut IRD Houel E. auteur aut IRD Gornitzka H. auteur aut IRD Fillaux J. auteur aut IRD Chevalley Séverine auteur aut IRD Valentin A. auteur aut IRD Jullian Valérie auteur aut IRD text journalArticle eng text/pdf born digital access We report the isolation and identification of a new quassinoid named simalikalactone E (SkE), extracted from a widely used Amazonian antimalarial remedy made out of Quassia amara L. (Simaroubaceae) leaves. This new molecule inhibited the growth of Plasmodium falciparum cultured in vitro by 50%, in the concentration range from 24 to 68 nM, independently of the strain sensitivity to chloroquine. We also showed that this compound was able to decrease gametocytemia with a 50% inhibitory concentration sevenfold lower than that of primaquine. SkE was found to be less toxic than simalikalactone D (SkD), another antimalarial quassinoid from Q. amara, and its cytotoxicity on mammalian cells was dependent on the cell line, displaying a good selectivity index when tested on nontumorogenic cells. In vivo, SkE inhibited murine malaria growth of Plasmodium vinckei petteri by 50% at 1 and 0.5 mg/kg of body weight/day, by the oral or intraperitoneal routes, respectively. The contribution of quassinoids as a source of antimalarial molecules needs therefore to be reconsidered. specialized 076 052 53 10 4393-4398 2009 0066-4804 https://www.documentation.ird.fr/hor/PAR00004140 10.1128/aac.00951-09 0066-4804 https://www.documentation.ird.fr/hor/PAR00004140 https://www.documentation.ird.fr/intranet/publi/2024-01/010086178.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2009-11-09 2024-01-04 PAR00004140 fre