@article{PAR00003863, title = {{A}naerobic degradation of landfill leachate using an upflow anaerobic fixed-bed reactor with microbial sulfate reduction}, author = {{B}en {D}hia {T}habet, {O}lfa and {B}ouallagui, {H}. and {C}ayol, {J}ean-luc and {O}llivier, {B}ernard and {F}ardeau, {M}arie-{L}aure and {H}amdi, {M}.}, editor = {}, language = {{ENG}}, abstract = {{T}his study evaluated the anaerobic degradation of landfill leachate and sulfate reduction as a function of {COD}/({SO}42-) ratio in an upflow anaerobic fixed-bed reactor. {T}he reactor, which was inoculated with a mixed consortium, was operated under a constant hydraulic retention time ({HRT}) of 5 days. {W}e investigated the effect of {COD}/({SO}42-) ratio variation on the sulfate reduction efficiency, hydrogen sulfide production, chemical oxygen demand ({COD}) removal, conductivity, and p{H} variation. {T}he best reactor performance, with significant sulfate reduction efficiency and {COD} removal efficiency of 91% and 87%. respectively, was reached under a {COD}/({SO}42-) ratio of 1.17. {U}nder these conditions, microscopic analysis showed the abundance of vibrios and rod-shaped bacterial cells. {T}wo anaerobic bacteria were isolated from the reactor sludge. {P}hylogenetic studies performed on these strains identified strain {A}1 as affiliated to {C}lostridium genus and strain {H}1 as a new species of sulfate-reducing bacteria affiliated to the {D}esulfovibrio genus. {T}he closest phylogenetic relative of strain {H}1 was {D}esulfovibrio desulfuricans, at 96% similarity for partial 16{S} {RNA} gene sequence data. {P}hysiological and metabolic characterization was performed for this strain.}, keywords = {{S}ulfate reduction ; {S}ulfate-reducing bacteria ; {H}ydrogen sulfide ; {D}esulfovibrio sp. ; {L}andfill leachate ; {A}naerobic bioreactor}, booktitle = {}, journal = {{J}ournal of {H}azardous {M}aterials}, volume = {167}, numero = {1-3}, pages = {1133--1140}, ISSN = {0304-3894}, year = {2009}, DOI = {10.1016/j.jhazmat.2009.01.114}, URL = {https://www.documentation.ird.fr/hor/{PAR}00003863}, }