@article{PAR00003347,
  title = {{I}nvolvement of peroxidases in the coffee resistance to orange rust ({H}emileia vastatrix)},
  author = {{S}ilva, {M}. {C}. and {G}uerra-{G}uimaraes, {L}. and {L}oureiro, {A}. and {N}icole, {M}ichel},
  editor = {},
  language = {{ENG}},
  abstract = {{C}offea arabica plants with the {S}({H})5 and {S}({H})4{S}({H})5 genotypes were inoculated with isolates of the coffee orange rust ({H}emileia vastatrix) races {II} and {VI}, in order to establish two incompatible interactions ({I}-1 and {I}-2) and a compatible one. {B}oth incompatible interactions were characterized by restricted fungal growth associated with rapid hypersensitive plant cell death, monitored by cell autofluorescence and/or browning. {C}ytological and biochemical studies were performed to investigate the association of peroxidases ({POD}s) with coffee resistance to rust. {I}n both incompatible interactions, in contrast with the compatible one, investigations revealed a peak of {POD} activity prior or at the same time, as the beginning of cell death. {D}uring the first peak, the isoenzyme pattern for peroxidases obtained by {IEF} (isoelectric focusing electrophoresis) showed an increase in activity of anionic and cationic isoenzymes. {C}ytochemically, {POD} and {H}2{O}2 were localized at the interface between the cuticle and fungal prepenetration structures, and at infection sites. {I}n both incompatible interactions, a later increase in {POD} activity was determined which can be related to host cell wall lignification. {T}his peak coincided with the one observed in the compatible interaction. {T}reatments of coffee leaves ({I}-2 incompatible interaction) with 2,4-dichlorophenol, an activator of peroxidases and other oxidases, significantly increased cell autofluorescence. {O}n the contrary, salicyl hydroxamic acid, an inhibitor of the same enzymes, and diphenyleneiodonium chloride, an inhibitor of {NADPH} oxidases, decreased cell autofluorescence. {T}hese results suggested that {POD}, {NADPH} oxidases and eventually other oxidases are involved in the coffee resistance to {H}. vastatrix.},
  keywords = {{C}offea arabica ; {H}emileia vastatrix ; {C}ytochemistry ; {B}iochemistry ; {R}esistance ; {H}ypersensitive reaction ; {P}eroxidases ; {H}2{O}2},
  booktitle = {},
  journal = {{P}hysiological and {M}olecular {P}lant {P}athology},
  volume = {72},
  numero = {1-3},
  pages = {29--38},
  ISSN = {0885-5765},
  year = {2008},
  DOI = {10.1016/j.pmpp.2008.04.004},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00003347},
}