%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Kim, H. H.
%A Lee, J. H.
%A Shin, D. J.
%A Ko, H. C.
%A Hwang, H. S.
%A Kim, T.
%A Cho, E. G.
%A Engelmann, Florent
%T Desiccation sensitivity and cryopreservation of korean ginseng seeds
%D 2008
%L PAR00003124
%G ENG
%J Cryo-Letters
%@ 0143-2044
%K After-ripening ; Desiccation ; Germination ; Panax ginseng ca meyer
%M ISI:000260405200007
%N 5
%P 419-426
%U https://www.documentation.ird.fr/hor/PAR00003124
%V 29
%W Horizon (IRD)
%X Korean ginseng germplasm is maintained as clonal germplasm since there is no practical method for long-term seed conservation. The aim of this study was to establish a cryopreservation protocol for Korean ginseng seeds. Desiccation of undehisced ginseng seeds to a moisture content (MC) of 7.1 % did not decrease their dehiscence and germination. After cryopreservation, the dehiscence percentage of desiccated seeds decreased for MC above 12.5%; it was 26% for 22.6% seed MC and nil for 41.9% seed MC. Germination percentage did not decrease significantly between 12.5-22.6% seed MC, while germination percentage of dehisced seeds decreased below 7.2% MC, reaching 25.8% at 3.8% MC. After cryopreservation, the germination percentage decreased from 90.5-92.9% at 8.3-10.6% MC to 84.8% at 12.5% MC. At MCs below 8.3%, germination rapidly decreased from 85.0% at 7.2% MC to 34.9% at 5.3% MC. Therefore, the hydration window for cryopreservation of ginseng seeds is around 8-11% MC. Undehisced Korean ginseng seeds were characterized by their high lipid and protein content (lipids, 42.6% FW; proteins, 41.0% FW). When using thermal analysis, during the cooling phase, exothermic ice crystallization peaks were observed with dehisced ginseng seeds above 13.5% MCs (3.3 J g(-1) FW). A second crystallization peak was detected following ice crystallization peaks.
%$ 076