Molecular phylogenetics of tsetse flies (Diptera : Glossinidae) based on mitochondrial (COI, 16S, ND2) and nuclear ribosomal DNA sequences, with an emphasis on the #palpalis$ group Dyer N. A. auteur aut IRD Lawton S. P. auteur aut IRD Ravel Sophie auteur aut IRD Choi K. S. auteur aut IRD Lehane M. J. auteur aut IRD Robinson A. S. auteur aut IRD Okedi L. M. auteur aut IRD Hall M. J. R. auteur aut IRD Solano Philippe auteur aut IRD Donnelly M. J. auteur aut IRD text journalArticle eng text/pdf born digital access Relationships of 13 species of the genus Glossina (tsetse flies) were inferred from mitochondrial (cytochrome oxidase 1, NADH dehydrogenase 2 and 16S) and nuclear (internal transcribed spacer 1 of rDNA) sequences. The resulting phylogeny confirms the monophyly of the morphologically defined fusca, morsitans and palpalis subgenera. Genetic distances between palpalis and morsitans subspecies suggest that their status needs revision. In Particular, cytochrome oxidose 1 sequences showed large geographical differences within G. palpalis palpalis, suggesting the existence of cryptic species within this subspecies. The morphology of palpalis group female genital plates was examined, and individuals were found varying outside the ranges specified by the standard identification keys, making definitive morphological classification impossible. A diagnostic PCR to distinguish G. palpalis palpalis, G. tachinoides and G. palpalis gambiensis based on length differences of internal transcribed spacer 1 sequences is presented. specialized Tsetse Glossina Diagnostic pcr Palpalis Cryptic species 052 020 Molecular Phylogenetics and Evolution 49 1 227-239 2008 1055-7903 https://www.documentation.ird.fr/hor/PAR00003085 10.1016/j.ympev.2008.07.011 1055-7903 [F B010044069] https://www.documentation.ird.fr/hor/PAR00003085 https://www.documentation.ird.fr/intranet/publi/2008/11/010044069.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2008-12-05 2017-08-23 PAR00003085 fre