@article{PAR00002703,
  title = {{C}ontrasting globulin and cysteine proteinase gene expression patterns reveal fundamental developmental differences between zygotic and somatic embryos of oil palm},
  author = {{A}berlenc {B}ertossi, {F}r{\'e}d{\'e}rique and {C}habrillange, {N}athalie and {D}uval, {Y}ves and {T}regear, {J}ames},
  editor = {},
  language = {{ENG}},
  abstract = {{O}il palm ({E}laeis guineensis {J}acq.) somatic embryos differ from zygotic embryos in that they accumulate only small amounts of storage proteins. {W}e compared the balance between deposition and degradation of storage proteins during zygotic or somatic embryogenesis and germinative growth in the two types of embryos. {D}uring mid to late zygotic embryogenesis, storage proteins accumulated and globulin 7{S} ({GLO}7{A}) gene transcripts were detected, whereas neither protease activity nor cysteine proteinase ({CPR}) gene transcripts were detected. {G}lobulin degradation occurred after 8 days of in vitro germination in zygotic embryos and was accompanied by a decrease in {GLO}7{A} transcripts. {T}ranscripts of three cysteine proteinase genes of the papain family were detected as early as {D}ay 2 of in vitro germination. {S}everal proteolytically active protein bands were identified by zymography, and {CPR}-like proteins were detected with an antibody raised against the {V}icia sativa {L}. cysteine proteinase {CPRI}. {P}rotease activities and {CPR}-like proteins were observed from {D}ay 8 onward when globulin degradation occurred. {D}uring somatic embryogenesis and subsequent germinative growth, only small amounts of storage proteins accumulated, even though {GLO}7{A} transcripts were detected. {T}wo of the three cysteine proteinase genes were expressed throughout both somatic embryogenesis and germinative growth. {P}rotease activities and {CPR}-like protein species were detected in somatic embryos at several developmental stages. {I}n contrast to zygotic embryogenesis, the accumulation of globulins and their subsequent mobilization appear to be concomitant processes during somatic embryogenesis, which could explain the low accumulation of storage proteins in somatic embryos.},
  keywords = {{E}laeis guineensis ; {E}mbryogenesis ; {G}ermination ; {P}rotease ; {S}torage protein},
  booktitle = {},
  journal = {{T}ree {P}hysiology},
  volume = {28},
  numero = {8},
  pages = {1157--1167},
  ISSN = {0829-318{X}},
  year = {2008},
  DOI = {10.1093/{TREEPHYS}/28.8.1157},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00002703},
}