@article{PAR00002416,
  title = {{A}grobacterium rhizogenes-transformed roots of coffee ({C}offea arabica) : {C}onditions for long-term proliferation, and morphological and molecular characterization},
  author = {{A}lpizar, {E}. and {D}echamp, {E}. and {L}apeyre {M}ontes, {F}. and {G}uilhaumon, {C}. and {B}ertrand, {B}. and {J}ourdan, {C}. and {L}ashermes, {P}hilippe and {E}tienne, {H}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground and {A}ims {T}he aims of this study were to set up proliferation conditions for hairy roots of {C}offea arabica regenerated after transformation by {A}grobacterium rhizogenes strain {A}4-{RS}, and to carry out the morphological and molecular characterization of hairy root clones maintained over the long term. {M}ethods {A}uxin supply, light conditions and sucrose concentration were modified with the aim of establishing efficient root proliferation conditions. {T}he morphological variability among 62 established hairy root clones was phenotyped by scanning the roots and analysing the images using 'whin{RHIZO}' software procedures. {PCR} analysis of integration in transformed root cells of rol and aux oncogenes from the {T}-{DNA} of the {R}i plasmid was used to study the molecular variability among clones. {K}ey {R}esults {A}uxin supply was necessary to obtain and stimulate growth and branching, and {IBA} applied at 0.5 mu {M} was the most efficient auxin. {S}ignificant differences were shown among the 62 clones for total root length and for the percentage of fine roots. {T}hese variables were stable across subcultures and could hence be used for efficient characterization of hairy root clones. {T}he majority of hairy root clones (86 %) exhibited non-significant phenotype differences with non-transformed roots. {E}ight clones were significantly different from the non-transformed controls in that they possessed a low proportion of fine roots. {T}wo other hairy root clones grew significantly faster than the other clones. {T}he {PCR} analysis revealed a low variability in the integration of rol and aux oncogenes in transformed root cells. {T}he {T}-{R}-{DNA} was never integrated as aux1 and aux2 genes were not found, although rol{B} and rol{C} genes from the {T}-{L}-{DNA} were always present. {C}onclusions {T}he discovery of low morphological variability among coffee hairy roots together with the identification of morphological variables allowing easy identification of phenotypically altered clones represent two important results. {T}hey make hairy roots a possible, and efficient, tool for functional-genomic studies of coffee root genes.},
  keywords = {{A}grobacterium rhizogenes ; aux genes ; {C}offea arabica ; genetic transformation ; hairy roots ; rol genes ; root morphology},
  booktitle = {},
  journal = {{A}nnals of {B}otany},
  volume = {101},
  numero = {7},
  pages = {929--940},
  ISSN = {0305-7364},
  year = {2008},
  DOI = {10.1093/aob/mcn027},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00002416},
}