Maïga O. auteur aut IRD Djimde A. A. auteur aut IRD Hubert V. auteur aut IRD Renard E. auteur aut IRD Aubouy Agnès auteur aut IRD Kironde F. auteur aut IRD Nsimba B. auteur aut IRD Koram K. auteur aut IRD Doumbo O. K. auteur aut IRD Bras J. auteur aut IRD Clain J. auteur aut IRD text journalArticle eng Background. Usefulness of sulfadoxine-pyrimethamine as first-line therapy for uncomplicated Plasmodium falciparum malaria and intermittent preventive treatment in pregnancy throughout sub-Saharan Africa is compromised by the spread of dhfr alleles associated with pyrimethamine resistance. A predominant haplotype associated with the N51I+C59R+S108N triple-mutant dhfr allele has been reported recently in 4 African countries. A more comprehensive picture of the evolution of this mutant allele in Africa is lacking. Methods. Seventy-five P. falciparum isolates carrying the wild-type dhfr allele and 204 carrying the triple-mutant dhfr allele from 11 African countries were selected. The genetic diversity of the chromosomes bearing these alleles was analyzed with 4 microsatellite markers closely linked to the dhfr gene. Results. Seventy-three different 4-locus haplotypes carrying the wild-type dhfr allele were found. By contrast, 175 ( 85%) of 204 isolates carrying the triple-mutant dhfr allele shared a unique haplotype, identical to the one identified in Thailand. For the remaining triple-mutant isolates and one isolate with the quadruple-mutant dhfr allele ( N51I+C59R+S108N+I164L), haplotypes were closely related to the predominant haplotype by mutation or recombination. Conclusions. Migration of parasites carrying an ancestral triple-mutant dhfr allele drives the spread of dhfr alleles associated with pyrimethamine resistance throughout West and Central Africa. specialized BENIN CAMEROUN CONGO COTE D'IVOIRE GABON GUINEE MALI SENEGAL OUGANDA COMORES 052 196 1 165-172 2007 0022-1899 https://www.documentation.ird.fr/hor/PAR00001707 10.1086/518512 0022-1899 [F B010069213] https://www.documentation.ird.fr/hor/PAR00001707 IRD - Base Horizon / Pleins textes 2007-09-04 2017-08-23 PAR00001707 fre