@article{PAR00001703,
  title = {{I}mproved assessment of {P}lasmodium vivax response to antimalarial drugs by a colorimetric double-site {P}lasmodium lactate dehydrogenase antigen capture enzyme-linked immunosorbent assay},
  author = {{D}ruilhe, {P}. and {B}rasseur, {P}hilippe and {B}lanc, {C}. and {M}akler, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he occurrence of {P}lasmodium vivax resistance to chloroquine has been reported in several countries of {A}sia and {S}outh {A}merica. {H}owever, the resistance of {P}. vivax is insufficiently documented for three reasons: it has received far less attention than {P}. falciparum; in vivo investigations are handicapped by the existence of hypnozoites, which make it difficult to distinguish between recrudescences due to drug failure and relapses due to dormant forms in the liver; and in vitro studies are greatly limited by the poor growth of {P}. vivax. {W}e report on the adaptation to {P}. vivax of a colorimetric double-site {P}lasmodium lactate dehydrogenase antigen capture enzyme-linked immunosorbent assay previously developed for {P}. falciparum. {T}he assay proved remarkably sensitive, as under optimal conditions it could detect {P}. vivax parasitemia levels as low as 10(-8). {T}he technique, which relies on the detection of protein synthesis by the parasite, yielded steep drug-response curves, leading to the precise determination of the 50% inhibitory concentrations for a high proportion of isolates. {C}hloroquine-resistant parasites were identified in an area where this phenomenon had been documented by in vivo methods. {T}hus, the results indicate that the in vitro susceptibility of {P}. vivax can now be monitored easily and efficiently. {T}he data suggest that the threshold of resistance is similar to that of {P}. falciparum, i.e., in the range of 100 n{M} for chloroquine and 15 n{M} for pyronaridine. {H}owever, further studies are required to precisely define the cutoff for resistance and the sensitivity to each drug.},
  keywords = {{AFRIQUE} {SUBSAHARIENNE}},
  booktitle = {},
  journal = {{A}ntimicrobial {A}gents and {C}hemotherapy},
  volume = {51},
  numero = {6},
  pages = {2112--2116},
  ISSN = {0066-4804},
  year = {2007},
  DOI = {10.1128/{AAC}.01385-06},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00001703},
}