@article{PAR00000418,
  title = {{V}ariability in response of seeds to liquid nitrogen exposure in wild coffee ({C}offea arabica {L}.)},
  author = {{V}asquez, {N}. and {S}alazar, {K}. and {A}nthony, {F}. and {C}habrillange, {N}. and {E}ngelmann, {F}. and {D}ussert, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n order to investigate the intraspecific variability for tolerance to liquid nitrogen ({LN}) exposure of coffee ({C}offea arabica) seeds, seed and embryo viability after desiccation or desiccation and cooling to -196 degrees {C}, with or without controlled rehydration of seeds after thawing, has been studied within a core subset of 30 accessions of the {CATIE} field genebank. {A} high variability for seed sensitivity to {LN} exposure was observed: seed viability after cryopreservation varied from 0 to 74% among the accessions studied (0 to 100% when expressed in percentage of the desiccation control) and was not correlated to seed size, seed water content after desiccation, or seed and embryo viability after desiccation. {B}y contrast, no variability was found between the genotypes studied for embryo viability after {LN} exposure. {V}iability of embryos after cryopreservation was always very high, indicating that, when occurring, the decline or loss of seed viability after {LN} exposure was due to damages to the endosperm only. {C}ontrolled post-thaw rehydration of seeds, through a 6-week osmoconditioning treatment with a -1.25 {MP}a polyethylene glycol solution, dramatically increased viability of frozen seeds. {H}owever, this effect was variable within the accessions studied and independent of seed viability after desiccation or desiccation and {LN} exposure.},
  keywords = {},
  booktitle = {},
  journal = {{S}eed {S}cience and {T}echnology},
  volume = {33},
  numero = {2},
  pages = {293--301},
  ISSN = {0251-0952},
  year = {2005},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00000418},
}