%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Snoeck, J.
%A Riva, C.
%A Steegen, K.
%A Schrooten, Y.
%A Maes, B.
%A Vergne, L.
%A Van Laethem, K.
%A Peeters, M.
%A Vandamme, A. M.
%T Optimization of a genotypic assay applicable to all human immunodeficiency virus type 1 protease and reverse transcriptase subtypes
%D 2005
%L PAR00000405
%G ENG
%J Journal of Virological Methods
%@ 0166-0934
%K genotypic assay ; RNase H region ; non B subtypes ; gag cleavage sites
%M CC:0002310552-0007
%N 1-2
%P 47-53
%R 10.1016/j.jviromet.2005.04.001
%U https://www.documentation.ird.fr/hor/PAR00000405
%V 128
%W Horizon (IRD)
%X Genotypic assays are used often to guide clinicians in decisions concerning the treatment of patients. An optimized sequence-based genotypic assay was used to determine the whole protease and reverse transcriptase (RT) gene, including the gag cleavage site region and RNase H region. Since non-B subtypes are increasing in countries where subtype B was the most prevalent Subtype, and treatment becomes more available in developing countries where the epidemic is characterized by a high prevalence of non-B subtypes, it was important that the genotypic test was evaluated using a panel of different subtypes. Amplification was successful for different subtypes: A, B, C, D, F, G, H, J, CRF01_AE, CRF02-AG, CRF11-cpx, CRF13-cpx and an uncharacterized recombinant sample. The detection limit of the PCR was 1000 copies/ml, except for 1 subtype C sample (PL3) and 1 CRF02_AG sample (PL8). The detection limit for these samples was 5000 copies/ml. A sequence could be obtained in both directions for most of the samples. (c) 2005 Elsevier B.V. All rights reserved.