@article{PAR00000405,
  title = {{O}ptimization of a genotypic assay applicable to all human immunodeficiency virus type 1 protease and reverse transcriptase subtypes},
  author = {{S}noeck, {J}. and {R}iva, {C}. and {S}teegen, {K}. and {S}chrooten, {Y}. and {M}aes, {B}. and {V}ergne, {L}. and {V}an {L}aethem, {K}. and {P}eeters, {M}. and {V}andamme, {A}. {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{G}enotypic assays are used often to guide clinicians in decisions concerning the treatment of patients. {A}n optimized sequence-based genotypic assay was used to determine the whole protease and reverse transcriptase ({RT}) gene, including the gag cleavage site region and {RN}ase {H} region. {S}ince non-{B} subtypes are increasing in countries where subtype {B} was the most prevalent {S}ubtype, and treatment becomes more available in developing countries where the epidemic is characterized by a high prevalence of non-{B} subtypes, it was important that the genotypic test was evaluated using a panel of different subtypes. {A}mplification was successful for different subtypes: {A}, {B}, {C}, {D}, {F}, {G}, {H}, {J}, {CRF}01_{AE}, {CRF}02-{AG}, {CRF}11-cpx, {CRF}13-cpx and an uncharacterized recombinant sample. {T}he detection limit of the {PCR} was 1000 copies/ml, except for 1 subtype {C} sample ({PL}3) and 1 {CRF}02_{AG} sample ({PL}8). {T}he detection limit for these samples was 5000 copies/ml. {A} sequence could be obtained in both directions for most of the samples. (c) 2005 {E}lsevier {B}.{V}. {A}ll rights reserved.},
  keywords = {genotypic assay ; {RN}ase {H} region ; non {B} subtypes ; gag cleavage sites},
  booktitle = {},
  journal = {{J}ournal of {V}irological {M}ethods},
  volume = {128},
  numero = {1-2},
  pages = {47--53},
  ISSN = {0166-0934},
  year = {2005},
  DOI = {10.1016/j.jviromet.2005.04.001},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00000405},
}