Snoeck J., Riva C., Steegen K., Schrooten Y., Maes B., Vergne L., Van Laethem K., Peeters M., Vandamme A. M. (2005). Optimization of a genotypic assay applicable to all human immunodeficiency virus type 1 protease and reverse transcriptase subtypes. Journal of Virological Methods, 128 (1-2), p. 47-53. ISSN 0166-0934.
Titre du document
Optimization of a genotypic assay applicable to all human immunodeficiency virus type 1 protease and reverse transcriptase subtypes
Snoeck J., Riva C., Steegen K., Schrooten Y., Maes B., Vergne L., Van Laethem K., Peeters M., Vandamme A. M.
Source
Journal of Virological Methods, 2005,
128 (1-2), p. 47-53 ISSN 0166-0934
Genotypic assays are used often to guide clinicians in decisions concerning the treatment of patients. An optimized sequence-based genotypic assay was used to determine the whole protease and reverse transcriptase (RT) gene, including the gag cleavage site region and RNase H region. Since non-B subtypes are increasing in countries where subtype B was the most prevalent Subtype, and treatment becomes more available in developing countries where the epidemic is characterized by a high prevalence of non-B subtypes, it was important that the genotypic test was evaluated using a panel of different subtypes. Amplification was successful for different subtypes: A, B, C, D, F, G, H, J, CRF01_AE, CRF02-AG, CRF11-cpx, CRF13-cpx and an uncharacterized recombinant sample. The detection limit of the PCR was 1000 copies/ml, except for 1 subtype C sample (PL3) and 1 CRF02_AG sample (PL8). The detection limit for these samples was 5000 copies/ml. A sequence could be obtained in both directions for most of the samples. (c) 2005 Elsevier B.V. All rights reserved.
