@article{PAR00000398,
  title = {{GAL}4-{GFP} enhancer trap lines for genetic manipulation of lateral root development in {A}rabidopsis thaliana},
  author = {{L}aplaze, {L}. and {P}arizot, {B}. and {B}aker, {A}. and {R}icaud, {L}. and {M}artiniere, {A}. and {A}uguy, {F}. and {F}ranche, {C}. and {N}ussaume, {L}. and {B}ogusz, {D}. and {H}aseloff, {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{L}ateral root development occurs throughout the life of the plant and is responsible for the plasticity of the root system. {I}n {A}rabidopsis thaliana, lateral root founder cells originate from pericycle cells adjacent to xylem poles. {I}n order to study the mechanisms of lateral root development, a population of {A}rabidopsis {GAL}4-{GFP} enhancer trap lines were screened and two lines were isolated with {GAL}4 expression in root xylem-pole pericycle cells ({J}0121), i.e. in cells competent to become lateral root founder cells, and in young lateral root primordia ({J}0192). {T}hese two enhancer trap lines are very useful tools with which to study the molecular and cellular bases of lateral root development using targeted gene expression. {T}hese lines were used for genetic ablation experiments by targeting the expression of a toxin-encoding gene. {M}oreover, the molecular bases of the enhancer trap expression pattern were characterized. {T}hese results suggest that the lateral-root-specific {GAL}4 expression pattern in {J}0192 is due to a strong enhancer in the promoter of the {LOB}-domain protein gene {LBD}16.},
  keywords = {genetic ablation ; lateral root founder cells ; lateral root primordia ; patterning ; pericycle},
  booktitle = {},
  journal = {{J}ournal of {E}xperimental {B}otany},
  volume = {56},
  numero = {419},
  pages = {2433--2442},
  ISSN = {0022-0957},
  year = {2005},
  DOI = {10.1093/jxb/eri236},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00000398},
}