@article{fdi:42259,
  title = {{E}stimating bacterial {DNA} synthesis from [3{H}] thymidine incorporation : discrepancies among macromolecular extraction procedures},
  author = {{T}orr{\'e}ton, {J}ean-{P}ascal and {B}ouvy, {M}arc},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he estimation of bacterial {DNA} synthesis in trophic studies with [3{H}] thymidine requires quantitative extraction of labeled {DNA}. {T}o determine the {DNA} contribution to total macromolecular labeling in a eutrophic ecosystem, we tested three extraction procedures currently used to estimate {DNA} labeling from thymidine incorportation : enzymatic fractionation, acid-base hydrolysis, and phenol-chloroform extraction. {B}ecause labeled macromolecular fractions are generally defined as {DNA}, {RNA}, and proteins, we used incorporation of tritiated thymidine, uridine, and leucine to preferentially label {DNA}, {RNA}, and proteins. {O}ur data showed that each fractionation method yielded different apparent macromolecular distributions of the same radiolabeled substrates. {E}nzymatic digestions of the fractions obtained by acid-base hydrolysis and phenol-chloroform extraction showed that these two procedures are inadequate for estimating bacterial {DNA} labeling in our ecosystem. {F}inally, using the enzymatic procedure at different sites, {DNA} labeling appeared to represent a nearly constant proportion of the labeled macromolecules (20,1%, r=0.952, n=101) over a wide range of incorporations rates. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{BACTERIE} ; {PRODUCTIVITE} {BIOLOGIQUE} ; {METHODE} {D}'{ANALYSE} ; {EXTRACTION} ; {HYDROLYSE} ; {FRACTIONNEMENT}},
  booktitle = {},
  journal = {{L}immnology and {O}ceanography},
  volume = {36},
  numero = {2},
  pages = {299--306},
  ISSN = {0024-3590},
  year = {1991},
  URL = {https://www.documentation.ird.fr/hor/fdi:42259},
}