%0 Journal Article %9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES %A Roussos, Sevastianos %A Raimbault, Maurice %A Saucedo-Castaneda, G. %A Lonsane, B.K. %T Efficient leaching of cellulases produced by Trichoderma harzianum in solid state fermentation %D 1992 %L fdi:39185 %G ENG %J Biotechnology Techniques %@ 0951-208X %K BIOTECHNOLOGIE ; FERMENTATION EN MILIEU SOLIDE ; MICROORGANISME ; EXTRACTION ; CELLULASE ; ACTIVITE ENZYMATIQUE ; CANNE A SUCRE ; BAGASSE ; METHODE %K PHASE LIQUIDE ; CHAMPIGNON FILAMENTEUX %N 5 %P 429-432 %R 10.1007/BF02447483 %U https://www.documentation.ird.fr/hor/fdi:39185 %> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_33-34/39185.pdf %V 6 %W Horizon (IRD) %X Recovery of cellulases from solid state culture of #Trichoderma harzianum$ was efficiently achieved by hydraulic pressing. Pressing of fermented solids yielded carboxymethyl-cellulase (CMCase) extraction efficiency of 71% and a ratio of leachate to fermented solids of 0.58 (v/w). Addition of water to pressed solids and second pressing improved the efficiency (95%) with simultaneous increase in the ratio to 1.16 (v/w). The overall extraction of filter paper activity was lower (85%) than that of CMCase. This technique is simple and its extraction efficiency is similar to that obtained in multiple-contact counter-current systems. The hydraulic press in its individuality was not used earlier to leach the product from fermented solids. (Résumé d'auteur) %$ 084FERMEN01