Roussos Sevastianos, Raimbault Maurice, Saucedo-Castaneda G., Lonsane B.K. (1992). Efficient leaching of cellulases produced by Trichoderma harzianum in solid state fermentation. Biotechnology Techniques, 6 (5), p. 429-432. ISSN 0951-208X.
Titre du document
Efficient leaching of cellulases produced by Trichoderma harzianum in solid state fermentation
Biotechnology Techniques, 1992,
6 (5), p. 429-432 ISSN 0951-208X
Recovery of cellulases from solid state culture of Trichoderma harzianum was efficiently achieved by hydraulic pressing. Pressing of fermented solids yielded carboxymethyl-cellulase (CMCase) extraction efficiency of 71% and a ratio of leachate to fermented solids of 0.58 (v/w). Addition of water to pressed solids and second pressing improved the efficiency (95%) with simultaneous increase in the ratio to 1.16 (v/w). The overall extraction of filter paper activity was lower (85%) than that of CMCase. This technique is simple and its extraction efficiency is similar to that obtained in multiple-contact counter-current systems. The hydraulic press in its individuality was not used earlier to leach the product from fermented solids. (Résumé d'auteur)
Plan de classement
Aérobies [084FERMEN01]
Descripteurs
BIOTECHNOLOGIE ; FERMENTATION EN MILIEU SOLIDE ; MICROORGANISME ; EXTRACTION ; CELLULASE ; ACTIVITE ENZYMATIQUE ; CANNE A SUCRE ; BAGASSE ; METHODE ; PHASE LIQUIDE ; CHAMPIGNON FILAMENTEUX
