Benavides Julio A., Godreuil S., Opazo-Capurro A., Mahamat O.O., Falcon N., Oravcova K., Streicker D.G., Shiva C. (2022). Long-term maintenance of multidrug-resistant Escherichia coli carried by vampire bats and shared with livestock in Peru. Science of The Total Environment, 810, 152045 [8 p.]. ISSN 0048-9697.
Titre du document
Long-term maintenance of multidrug-resistant Escherichia coli carried by vampire bats and shared with livestock in Peru
Année de publication
2022
Auteurs
Benavides Julio A., Godreuil S., Opazo-Capurro A., Mahamat O.O., Falcon N., Oravcova K., Streicker D.G., Shiva C.
Source
Science of The Total Environment, 2022,
810, 152045 [8 p.] ISSN 0048-9697
Extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-E. coli) have been reported in wildlife worldwide. Whether wildlife is a transient host of ESBL-E. coli or comprises an independently maintained reservoir is unknown. We investigated this question by longitudinally monitoring ESBL-E. coli in common vampire bats and nearby livestock in Peru. Among 388 bats from five vampire bat colonies collected over three years, ESBL-E. coli were detected at a low prevalence (10% in 2015, 4% in 2017 and 2018) compared to a high prevalence (48%) from 134 livestock sampled in 2017. All ESBL-E. coli were multidrug-resistant, and whole genome sequencing of 33 randomly selected ESBL-E. coli isolates (18 recovered from bats) detected 46 genes conferring resistance to antibiotics including third-generation cephalosporins (e.g., blaCTX-M-55, blaCTX-M-15, blaCTX-M-65, blaCTX-M-3, blaCTX-M-14), aminoglycosides, fluoroquinolones, and colistin (mcr-1).
The mcr-1 gene is reported for the first time on a wild bat in Latin America. ESBL-E. coli also carried 31 plasmid replicon types and 16 virulence genes. Twenty-three E. coli sequence types (STs) were detected, including STs involved in clinical infections worldwide (e.g., ST 167, ST 117, ST 10, ST 156 and ST 648). ESBL-E. coli with identical cgMLST (ST 167) were detected in the same bat roost in 2015 and 2017, and several ESBL-E. coli from different bat roosts clustered together in the cgMLST reconstruction, suggesting long-term maintenance of ESBL-E. coli within bats. Most antibiotic resistance and virulence genes were detected in E. coli from both host populations, while ESBL-E. coli ST 744 was found in a bat and a pig from the same locality, suggesting possible cross-species exchanges of genetic material and/or bacteria between bats and livestock. This study suggests that wild mammals can maintain multidrug-resistant bacteria and share them with livestock.
Plan de classement
Epidémiologie générale [050EPID]
;
Maladies des animaux [080PROANI05]
Description Géographique
PEROU
Localisation
Fonds IRD [F B010096630]
Identifiant IRD
fdi:010096630
