@article{fdi:010090359,
  title = {{E}ffect of enzyme addition on fermentative hydrogen production from wheat straw},
  author = {{Q}u{\'e}m{\'e}neur, {M}arianne and {B}ittel, {M}. and {T}rably, {E}. and {D}umas, {C}. and {F}ourage, {L}. and {R}avot, {G}. and {S}teyer, {J}.{P}. and {C}arr{\`e}re, {H}.},
  editor = {},
  language = {{ENG}},
  abstract = {{W}heat straw is an abundant agricultural residue which can be used as raw material to produce hydrogen ({H}-2), a promising alternative energy carrier, at a low cost. {B}ioconversion of lignocellulosic biomass to produce {H}-2 usually involves three main operations: pretreatment, hydrolysis and fermentation. {I}n this study, the efficiency of exogenous enzyme addition on fermentative {H}-2 production from wheat straw was evaluated using mixed-cultures in two experimental systems: a one-stage system (direct enzyme addition) and a two-stage system (enzymatic hydrolysis prior to dark fermentation). {H}-2 production from untreated wheat straw ranged from 5.18 to 10.52 m{L}-{H}-2 g-{VS}-1. {W}hatever the experimental enzyme addition procedure, a two-fold increase in {H}-2 production yields ranging from 11.06 to 19.63 m{L}-{H}-2 g-{VS}-1 was observed after enzymatic treatment of the wheat straw. {T}he high variability in {H}-2 yields in the two step process was explained by the consumption of free sugars by indigenous wheat straw microorganisms during enzymatic hydrolysis. {T}he direct addition of exogenous enzymes in the one-stage dark fermentation stage proved to be the best way of significantly improving {H}-2 production from lignocellulosic biomass. {F}inally, the optimal dose of enzyme mixture added to the wheat straw was evaluated between 1 and 5 mg-protein g-raw wheat straw(-1)},
  keywords = {},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {H}ydrogen {E}nergy},
  volume = {37},
  numero = {14},
  pages = {10639--10647},
  ISSN = {0360-3199},
  year = {2012},
  DOI = {10.1016/j.ijhydene.2012.04.083},
  URL = {https://www.documentation.ird.fr/hor/fdi:010090359},
}