%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Quéméneur, Marianne
%A Hamelin, J.
%A Latrille, E.
%A Steyer, J.P.
%A Trably, E.
%T Functional versus phylogenetic fingerprint analyses for monitoring hydrogen-producing bacterial populations in dark fermentation cultures
%D 2011
%L fdi:010090358
%G ENG
%J International Journal of Hydrogen Energy
%@ 0360-3199
%M ISI:000289331800010
%N 6
%P 3870-3879
%R 10.1016/j.ijhydene.2010.12.100
%U https://www.documentation.ird.fr/hor/fdi:010090358
%> https://www.documentation.ird.fr/intranet/publi/2023-09/010090358.pdf
%V 36
%W Horizon (IRD)
%X The fermentative hydrogen production occurs during the anaerobic digestion of organic matter. It is a promising technology to produce renewable energy. However, the mixed culture fermentation performances vary considerably depending on the operating conditions such as pH. We investigated the potential of a molecular CE-SSCP (capillary electrophoresis-single strand conformation polymorphism) fingerprinting method based on the hydA functional genes to better describe the bacterial community dynamics, with regards to the standard 16S rDNA-based method. A series of batch experiments was performed from sucrose at different initial pH from 4 to 6. As expected, the highest H-2 production potentials (H-max) and rates (R-max) were obtained at the highest pH. Changes in batch performances were clearly associated with shifts in the hydA diversity and structure. In contrast, 16S rDNA-based fingerprints were less sensitive to changes in H-2 production performances. The H-max was related to lower hydA diversity, with Clostridium sporogenes as the major H-2 producer. Communities harboring larger hydA diversities were found in experiments with the higher R-max, suggesting that species coexistence may have positive effects on H-2 production.
%$ 084FERMEN02