@article{fdi:010090358,
  title = {{F}unctional versus phylogenetic fingerprint analyses for monitoring hydrogen-producing bacterial populations in dark fermentation cultures},
  author = {{Q}u{\'e}m{\'e}neur, {M}arianne and {H}amelin, {J}. and {L}atrille, {E}. and {S}teyer, {J}.{P}. and {T}rably, {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he fermentative hydrogen production occurs during the anaerobic digestion of organic matter. {I}t is a promising technology to produce renewable energy. {H}owever, the mixed culture fermentation performances vary considerably depending on the operating conditions such as p{H}. {W}e investigated the potential of a molecular {CE}-{SSCP} (capillary electrophoresis-single strand conformation polymorphism) fingerprinting method based on the hyd{A} functional genes to better describe the bacterial community dynamics, with regards to the standard 16{S} r{DNA}-based method. {A} series of batch experiments was performed from sucrose at different initial p{H} from 4 to 6. {A}s expected, the highest {H}-2 production potentials ({H}-max) and rates ({R}-max) were obtained at the highest p{H}. {C}hanges in batch performances were clearly associated with shifts in the hyd{A} diversity and structure. {I}n contrast, 16{S} r{DNA}-based fingerprints were less sensitive to changes in {H}-2 production performances. {T}he {H}-max was related to lower hyd{A} diversity, with {C}lostridium sporogenes as the major {H}-2 producer. {C}ommunities harboring larger hyd{A} diversities were found in experiments with the higher {R}-max, suggesting that species coexistence may have positive effects on {H}-2 production.},
  keywords = {},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {H}ydrogen {E}nergy},
  volume = {36},
  numero = {6},
  pages = {3870--3879},
  ISSN = {0360-3199},
  year = {2011},
  DOI = {10.1016/j.ijhydene.2010.12.100},
  URL = {https://www.documentation.ird.fr/hor/fdi:010090358},
}