@article{fdi:010088681,
  title = {{A}ssessing the diversity of plankton-associated prokaryotes along a size-fraction gradient : a methodological evaluation},
  author = {{C}abrol, {L}{\'e}a and {D}elleuze, {M}. and {S}zylit, {A}. and {S}chwob, {G}. and {Q}u{\'e}m{\'e}neur, {M}arianne and {M}isson, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{M}arine free-living ({FL}) and plankton-associated prokaryotes (plankton-microbiota) are at the basis of trophic webs and play crucial roles in the transfer and cycling of nutrients, organic matter, and contaminants. {D}ifferent ecological niches exist along the plankton size fraction gradient. {D}espite its relevant ecological role, the plankton-microbiota has rarely been investigated with a sufficient level of size-fraction resolution, and it can be challenging to study because of overwhelming eukaryotic {DNA}. {H}ere we compared the prokaryotic diversity obtained by 16{S} r{RNA} gene sequencing from six plankton size fractions (from {FL} to mesoplankton), through three {DNA} recovery methods: direct extraction, desorption pretreatment, enrichment post-treatment. {T}he plankton microbiota differed strongly according to the plankton size-fraction and methodological approach. {P}rokaryotic taxa specific to each size fraction, and methodology used, were identified. {V}ibrionaceae were over-represented by cell desorption pretreatment, while prokaryotic {DNA} enrichment had taxon-specific effects, indicating that direct {DNA} extraction was the most appropriate method.},
  keywords = {{P}lankton size fraction ; {DNA} extraction method ; {C}ell desorption ; {P}rokaryotic {DNA} enrichment ; {V}ibrio ; {E}ukaryotic {DNA} depletion ; {MEDITERRANEE}},
  booktitle = {},
  journal = {{M}arine {P}ollution {B}ulletin},
  volume = {197},
  numero = {},
  pages = {115688 [11 ]},
  ISSN = {0025-326{X}},
  year = {2023},
  DOI = {10.1016/j.marpolbul.2023.115688},
  URL = {https://www.documentation.ird.fr/hor/fdi:010088681},
}