@article{fdi:010087888,
  title = {{S}tructural variation turnovers and defective genomes : key drivers for the in vitro evolution of the large double-stranded {DNA} koi herpesvirus ({KHV})},
  author = {{N}ovelia {F}uandila, {N}. and {G}osselin-{G}renet, {A}.{S}. and {T}ilak, {M}.{K}. and {B}ergmann, {S}.{V}. and {E}scoubas, {J}.{M}. and {K}lafack, {S}. and {L}usiastuti, {A}.{M}. and {Y}uhana, {M}. and {F}iston-{L}avier, {A}.{S}. and {A}varre, {J}ean-{C}hristophe and {C}herif, {E}mira},
  editor = {},
  language = {{ENG}},
  abstract = {{S}tructural variations ({SV}s) constitute a significant source of genetic variability in virus genomes. {Y}et knowledge about {SV} variability and contribution to the evolutionary process in large double-stranded (ds){DNA} viruses is limited. {C}yprinid herpesvirus 3 ({C}y{HV}-3), also commonly known as koi herpesvirus({KHV}), has the largest ds{DNA} genome within herpesviruses. {T}his virus has become one of the biggest threats to common carp and koi farming, resulting in high morbidity and mortalities of fishes, serious environmental damage, and severe economic losses. {A} previous study analyzing {C}y{HV}-3 virulence evolution during serial passages onto carp cell cultures suggested that {C}y{HV}-3 evolves, at least in vitro, through an assembly of haplotypes that alternatively become dominant or under-represented. {T}he present study investigates the {SV} diversity and dynamics in {C}y{HV}-3 genome during 99 serial passages in cell culture using, for the first time, ultra-deep whole-genome and amplicon-based sequencing. {T}he results indicate that {KHV} polymorphism mostly involves {SV}s. {T}hese {SV}s display a wide distribution along the genome and exhibit high turnover dynamics with a clear bias towards inversion and deletion events. {A}nalysis of the pathogenesis-associated {ORF}150 region in ten intermediate cell passages highlighted mainly deletion, inversion and insertion variations that deeply altered the structure of {ORF}150. {O}ur findings indicate that {SV} turnovers and defective genomes represent key drivers in the viral population dynamics and in vitro evolution of {KHV}. {T}hus, the present study can contribute to the basic research needed to design safe live-attenuated vaccines, classically obtained by viral attenuation after serial passages in cell culture},
  keywords = {},
  booktitle = {},
  journal = {{P}eer {C}ommunity {J}ournal},
  volume = {2},
  numero = {},
  pages = {e44. [14 ]},
  year = {2022},
  DOI = {10.24072/pcjournal.154/},
  URL = {https://www.documentation.ird.fr/hor/fdi:010087888},
}