Publications des scientifiques de l'IRD

Ngnindji-Youdje Y., Diarra Adama Zan, Lontsi-Demano M., Tchuinkam T., Parola P. (2022). Detection of tick-borne pathogens in ticks from cattle in western highlands of Cameroon. Microorganisms, 10 (10), 1957 [15 p.].

Titre du document
Detection of tick-borne pathogens in ticks from cattle in western highlands of Cameroon
Année de publication
2022
Type de document
Article référencé dans le Web of Science WOS:000873181800001
Auteurs
Ngnindji-Youdje Y., Diarra Adama Zan, Lontsi-Demano M., Tchuinkam T., Parola P.
Source
Microorganisms, 2022, 10 (10), 1957 [15 p.]
This study aimed to detect and identify microorganisms in ticks collected in the Western Highlands of Cameroon. Quantitative real-time and standard PCR assays, coupled with sequencing, were used. A total of 944 ticks collected from cattle in five distinct sites in Cameroon were selected for the analyses. They belonged to five genera (Amblyomma, Hyalomma, Rhipicephalus, Haemaphysalis, and Ixodes) and twelve species. Real-time PCR revealed that 23% (n = 218) of the ticks were positive for Rickettsia spp., 15% (n = 141) for bacteria of the Anaplasmataceae family, 3% (n = 29) for Piroplasmida, 0.5% (n = 5) for Coxiella burnetii, 0.4% (n = 4) for Borrelia spp., and 0.2% (n = 2) for Bartonella spp. The co-infection rate (3.4%, n = 32) involved mainly Rickettsia spp. and Anaplasmataceae. Of the Rickettsia spp. positive ticks, the targeted PCR and sequencing yielded Rickettsia africae (78.9%), Rickettsia aeschlimannii (6.4%), Rickettsia massiliae (7.8%), Candidatus Rickettsia barbariae (0.9%), and Rickettsia sp. (0.9%). Anaplasmataceae included Anaplasma marginale (4.3%), Anaplasma platys (1.4%), Anaplasma centrale (0.7%), Ehrlichia ruminantium (0.7%), Wolbachia sp., Candidatus Ehrlichia rustica (13.5%), Candidatus Ehrlichia urmitei (7%), and an uncultured Ehrlichia sp. (4.2%). Borrelia theileri was identified in one Rhipicephalus microplus tick. Unfortunately, Piroplasmida could not be identified to the species level. This study demonstrates that in Cameroon, ticks harbour a wide variety of microorganisms and present a risk of zoonotic diseases.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052] ; Sciences du monde animal [080]
Description Géographique
CAMEROUN
Localisation
Fonds IRD [F B010086421]
Identifiant IRD
fdi:010086421
Contact