@article{fdi:010086405,
  title = {{K}inetics of monocyte subpopulations during experimental cerebral malaria and its resolution in a model of late chloroquine treatment},
  author = {{R}oyo, {J}ade and {C}amara, {A}. and {B}ertrand, {B}. and {B}atigne, {P}. and {C}oste, {A}. and {P}ipy, {B}. and {A}ubouy, {A}gn{\`e}s and {N}euro{CM} {G}roup},
  editor = {},
  language = {{ENG}},
  abstract = {{C}erebral malaria ({CM}) is one of the most severe forms of malaria and is a neuropathology that can lead to death. {M}onocytes have been shown to accumulate in the brain microvasculature at the onset of neurological symptoms during {CM}. {M}onocytes have a remarkable ability to adapt their function to their microenvironment from pro-inflammatory to resolving activities. {T}his study aimed to describe the behavior of monocyte subpopulations during infection and its resolution. {C}57{BL}/6 mice were infected with the {P}lasmodium berghei {ANKA} strain and treated or not with chloroquine ({CQ}) on the first day of the onset of neurological symptoms (day 6) for 4 days and followed until day 12 to mimic neuroinflammation and its resolution during experimental {CM}. {L}y6{C} monocyte subpopulations were identified by flow cytometry of cells from the spleen, peripheral blood, and brain and then quantified and characterized at different time points. {I}n the brain, the {L}y6{C}(int) and {L}y6{C}(low) monocytes were associated with neuroinflammation, while {L}y6{C}(hi) and {L}y6{C}(int) were mobilized from the peripheral blood to the brain for resolution. {D}uring neuroinflammation, {CD}36 and {CD}163 were both involved via splenic monocytes, whereas our results suggest that the low {CD}36 expression in the brain during the neuroinflammation phase was due to degradation. {T}he resolution phase was characterized by increased expressions of {CD}36 and {CD}163 in blood {L}y6{C}(low) monocytes, a higher expression of {CD}36 in the microglia, and restored high expression levels of {CD}163 in {L}y6{C}(hi) monocytes localized in the brain. {T}hus, our results suggest that increasing the expressions of {CD}36 and {CD}163 specifically in the brain during the neuroinflammatory phase contributes to its resolution.},
  keywords = {cerebral malaria ({CM}) ; mice ; monocyte ; brain ; spleen ; blood ; chloroquine},
  booktitle = {},
  journal = {{F}rontiers in {C}ellular and {I}nfection {M}icrobiology},
  volume = {12},
  numero = {},
  pages = {952993 [12 ]},
  ISSN = {2235-2988},
  year = {2022},
  DOI = {10.3389/fcimb.2022.952993},
  URL = {https://www.documentation.ird.fr/hor/fdi:010086405},
}