Dubelaar G.B.J., Casotti R., Tarran G.A., Biegala Isabelle. (2007). Phytoplankton and their analysis by flow cytometry. In :
Dolezel J. (ed.), Greilhuber J. (ed.), Suda J. (ed.). Flow cytometry with plant cells : analysis of genes, chromosomes and genomes. Weinheim : Wiley-VCH, 287-322. ISBN 978-3-527-31487-4.
Titre du document
Phytoplankton and their analysis by flow cytometry
Année de publication
2007
Type de document
Partie d'ouvrage
Auteurs
Dubelaar G.B.J., Casotti R., Tarran G.A., Biegala Isabelle
In
Dolezel J. (ed.), Greilhuber J. (ed.), Suda J. (ed.), Flow cytometry with plant cells : analysis of genes, chromosomes and genomes
Source
Weinheim : Wiley-VCH, 2007,
287-322 ISBN 978-3-527-31487-4
This chapter outlines how flow cytometry can be used for the analysis of phytoplankton : from basic and straightforward analysis to more challenging applications.Whereas most applications of flow cytometry in aquatic science are still laboratory-based, the emphasis of this chapter is on its potential to be used in situ, operated on a high frequency basis. This permits high-resolution sampling in time and space, which is crucial for our understanding of aquatic microbial ecosystems. Beginning with a basic description of the target particles, a sketch is made of the aquatic environment of these microorganisms and specific properties of the environment that may be very different from typical biomedical or plant research conditions. Some phytoplankton-related limitations and pitfalls are discussed as well as special instruments and instrument modification for phytoplankton analysis. Phytoplankton sampling is described with consideration to 'critical scales', including the use of platforms such as research ships and ships of opportunity, submerged use in vertical casts or on autonomous underwater vehicles and moored platforms. Various applications are presented, including the screening of species, probing phytoplankton biodiversity, monitoring 'harmful algal blooms', studying population-related processes, and cell-related processes and functioning. Plankton abundance patterns in the sea are assessed and the coupling of flow cytometry data to ocean optics and physics is advocated. Finally, the potential use of flow cytometry in protection and warning is discussed, including the monitoring of harmful algal blooms, water quality in the aquaculture industry, bathing waters and the drinking water industry, as well as with bio-indicatiors and the control of ship's ballast water treatment systems.
Plan de classement
Limnologie biologique / Océanographie biologique [034]
;
Ecologie, systèmes aquatiques [036]
Localisation
Fonds IRD [F B010085834]
Identifiant IRD
fdi:010085834
