@article{fdi:010085707, title = {{E}ffect of {A}zospirillum brasilense inoculation on rhizobacterial communities analyzed by denaturing gradient gel electrophoresis and automated ribosomal intergenic spacer analysis}, author = {{L}erner, {A}. and {H}erschkovitz, {Y}. and {B}audoin, {E}z{\'e}kiel and {N}azaret, {S}. and {M}o{\¨e}nne-{L}occoz, {Y}. and {O}kon, {Y}. and {J}urkevitch, {E}.}, editor = {}, language = {{ENG}}, abstract = {{N}ucleic acid-based techniques allow the exploration of microbial communities in the environments such as the rhizosphere. {A}zospirillum brasilense, a plant growth promoting rhizobacterium ({PGPR}), causes morphological changes in the plant root system. {T}hese changes in root physiology may indirectly affect the microbial diversity of the rhizosphere. {I}n this study, the changes in the rhizobacterial structure following {A}. brasilense inoculation of maize ({Z}ea mays) plants was examined by {PCR}-denaturating gradient gel electrophoresis ({DGGE}) and automated ribosomal intergenic spacer analysis ({ARISA}), using two universal primers sets for the 16{S} r{RNA} gene, and an intergenic 16{S}-23{S} r{DNA} primer set, respectively. {S}imilar results were obtained when using either {ARISA} or {DGGE} performed with these different primer sets, and analyzed by different statistical methods: no prominent effect of {A}. brasilense inoculation was observed on the bacterial communities of plant roots grown in two different soils and in different growth systems. {I}n contrast, plant age caused significant shifts in the bacterial populations.}, keywords = {{FRANCE}}, booktitle = {}, journal = {{S}oil {B}iology and {B}iochemistry}, volume = {38}, numero = {6}, pages = {1212--1218}, ISSN = {0038-0717}, year = {2006}, DOI = {10.1016/j.soilbio.2005.10.007}, URL = {https://www.documentation.ird.fr/hor/fdi:010085707}, }