@article{fdi:010085705,
  title = {{D}evelopment of a real-time {PCR} method to quantify the {PGPR} strain {A}zospirillum lipoferum {CRT}1 on maize seedlings},
  author = {{C}ouillerot, {O}. and {B}ouffaud, {M}.{L}. and {B}audoin, {E}z{\'e}kiel and {M}uller, {D}. and {C}aballero-{M}ellado, {J}. and {M}o{\¨e}nne-{L}occoz, {Y}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}zospirillum lipoferum {CRT}1 is a promising phytostimulatory {PGPR} for maize, whose effect on the plant is cell density-dependent. {A} nested {PCR} method is available for detection of the strain but does not allow quantification. {T}he objective was to develop a real-time {PCR} method for quantification of {A}. lipoferum {CRT}1 in the rhizosphere of maize seedlings. {P}rimers were designed based on a strain-specific {RFLP} marker, and their specificity was verified under qualitative and quantitative {PCR} conditions based on successful {CRT}1 amplification and absence of cross-reaction with genomic {DNA} from various rhizosphere strains. {R}eal-time {PCR} conditions were then optimized using {DNA} from inoculated or non-inoculated maize rhizosphere samples. {T}he detection limit was 60 fg {DNA} (corresponding to 19 cells) with pure cultures and 4 x 10(4) {CFU} equivalents g(-1) lyophilized sample consisting of mixture of rhizosphere soil and roots. {I}noculant quantification was effective down to 10(4) {CFU} equivalents g(-1). {A}ssessment of {CRT}1 rhizosphere levels in a field trial was in accordance with estimates from semi-quantitative {PCR} targeting another locus. {T}his real-time {PCR} method, which is now available for direct rhizosphere monitoring of {A}. lipoferum {CRT}1 in greenhouse and field experiments, could be used as a reference for developing quantification tools for other {A}zospirillum inoculants.},
  keywords = {{ALLEMAGNE} ; {FRANCE} ; {DUREN} ; {SAINT} {ANDRE} {COTE}},
  booktitle = {},
  journal = {{S}oil {B}iology and {B}iochemistry},
  volume = {42},
  numero = {12},
  pages = {2298--2305},
  ISSN = {0038-0717},
  year = {2010},
  DOI = {10.1016/j.soilbio.2010.09.003},
  URL = {https://www.documentation.ird.fr/hor/fdi:010085705},
}