@article{fdi:010085369,
  title = {{M}olecular characterization of some equine vector-borne diseases and associated arthropods in {E}gypt},
  author = {{A}bdulla, {H}. {H}. {A}. {M}. and {A}boelsoued, {D}. and {F}ang, {T}. {K}. and {A}bdel-{S}haft, {S}. and {M}egeed, {K}. {N}. {A}. and {P}arola, {P}. and {R}aoult, {D}. and {M}ediannikov, {O}leg},
  editor = {},
  language = {{ENG}},
  abstract = {{E}quine vector-borne diseases ({EVBD}s) are emerging and re-emerging diseases, and most of them are zoonotic. {T}his study aimed to investigate {EVBD}s in equines and associated arthropods (ticks and flies) from {E}gypt using molecular analyses, in addition to a preliminary characterization of associated ticks and flies by the matrixassisted laser desorption/ionization time of flight ({MALDI}-{TOF}) and molecular techniques. {I}n this study, 335 blood samples were obtained from equines that appeared to be in good health (320 horses and 15 donkeys) in {C}airo and {B}eni {S}uef provinces, {E}gypt. {F}rom the same animals, 166 arthropods (105 sucking flies and 61 ticks) were collected. {T}icks and flies were preliminary characterized by the {MALDI}-{TOF} and molecular tools. {Q}uantitative {PCR} (q{PCR}) and standard {PCR} coupled with sequencing were performed on the {DNA} of equines, ticks, and flies to screen multiple pathogens. {T}he {MALDI}-{TOF} and molecular characterization of arthropods revealed that louse fly ({H}ippobosca equina) and cattle tick ({R}hipicephalus annulatus) infesting equines. {A}naplasma platys-like (1.6%), {A}naplasma marginale (1.6%), {C}andidatus {E}hrlichia rustica (6.6%), a new {E}hrlichia sp. (4.9%), and {B}orrelia theileri (3.3%) were identified in {R}. annulatus. {A}naplasma sp. and {B}orrelia sp. {DNA}s were only detected in {H}. equina by q{PCR}. {A}. marginale, {A}naplasma ovis, and {T}heileria ovis recorded the same low infection rate (0.6%) in donkeys, while horses were found to be infected with {T}heileria equi and a new {T}heileria sp. {A}frica with recorded prevalence rates of 1.2% and 2.7%, respectively. {I}n conclusion, different pathogens were first detected such as {A}. platys-like, {C}andidatus {E}. rustica, and a new {E}hrlichia sp. in {R}. annulatus; {A}. marginale, {A}. ovis, and {T}. ovis in donkeys; and a new {T}heileria sp. "{A}frica" in horses.},
  keywords = {{E}quine ; {H}ippobosca equina ; {MALDI}-{TOF} ; {PCR} ; {R}hipicephalus annulatus ; {V}ector-borne diseases ; {EGYPTE}},
  booktitle = {},
  journal = {{A}cta {T}ropica},
  volume = {227},
  numero = {},
  pages = {106274 [13 ]},
  ISSN = {0001-706{X}},
  year = {2022},
  DOI = {10.1016/j.actatropica.2021.106274},
  URL = {https://www.documentation.ird.fr/hor/fdi:010085369},
}