@article{fdi:010085204,
  title = {{T}he rice {ILI}2 locus is a bidirectional target of the {A}frican {X}anthomonas oryzae pv. oryzae major transcription activator-like effector {T}al{C} but does not contribute to disease susceptibility},
  author = {{D}oucoure, {H}. and {A}uguy, {F}lorence and {B}lanvillain-{B}aufume, {S}. and {F}abre, {S}. and {G}abriel, {M}. and {T}homas, {E}. and {D}ambreville, {F}. and {S}ciallano, {C}. and {S}zurek, {B}oris and {K}oita, {O}. and {V}erdier, {V}al{\'e}rie and {C}unnac, {S}{\'e}bastien},
  editor = {},
  language = {{ENG}},
  abstract = {{X}anthomonas oryzae pv. oryzae ({X}oo) strains that cause bacterial leaf blight ({BLB}) limit rice ({O}ryza sativa) production and require breeding more resistant varieties. {T}ranscription activator-like effectors ({TALE}s) activate transcription to promote leaf colonization by binding to specific plant host {DNA} sequences termed effector binding elements ({EBE}s). {X}oo major {TALE}s universally target susceptibility genes of the {SWEET} transporter family. {TALE}-unresponsive alleles of clade {III} {O}s{SWEET} susceptibility gene promoter created with genome editing confer broad resistance on {A}sian {X}oo strains. {A}frican {X}oo strains rely primarily on the major {TALE} {T}al{C}, which targets {O}s{SWEET}14. {A}lthough the virulence of a tal{C} mutant strain is severely impaired, abrogating {O}s{SWEET}14 induction with genome editing does not confer equivalent resistance on {A}frican {X}oo. {T}o address this contradiction, we postulated the existence of a {T}al{C} target susceptibility gene redundant with {O}s{SWEET}14. {B}ioinformatics analysis identified a rice locus named {ATAC} composed of the {INCREASED} {LEAF} {INCLINATION} 2 ({ILI}2) gene and a putative lnc{RNA} that are shown to be bidirectionally upregulated in a {T}al{C}-dependent fashion. {G}ain-of-function approaches with designer {TALE}s inducing {ATAC} sequences did not complement the virulence of a {X}oo strain defective for {SWEET} gene activation. {W}hile editing the {T}al{C} {EBE} at the {ATAC} loci compromised {T}al{C}-mediated induction, multiplex edited lines with mutations at the {O}s{SWEET}14 and {ATAC} loci remained essentially susceptible to {A}frican {X}oo strains. {O}verall, this work indicates that {ATAC} is a probable {T}al{C} off-target locus but nonetheless documents the first example of divergent transcription activation by a native {TALE} during infection.},
  keywords = {rice ; {X}anthomonas oryzae ; {TAL} effector ; disease},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {M}olecular {S}ciences},
  volume = {23},
  numero = {10},
  pages = {5559 [20 ]},
  year = {2022},
  DOI = {10.3390/ijms23105559},
  URL = {https://www.documentation.ird.fr/hor/fdi:010085204},
}