@article{fdi:010084540, title = {{F}ast and novel botanical exploration of a 320-km transect in eastern {A}mazonia using {DNA} barcoding}, author = {{M}illiken, {W}. and {O}donne, {G}. and {E}ngel, {J}ulien and {L}e {T}ourneau, {F}. {M}. and {S}uescun, {U}. and {C}have, {J}.}, editor = {}, language = {{ENG}}, abstract = {{W}e explored a 320-km transect in the {T}umucumaque mountain range along the border between southern {F}rench {G}uiana and {B}razil, sampling all trees and lianas with {DBH} >= 10 cm in seven 25 x 25-m plots installed near seven boundary milestones. {W}e isolated {DNA} from cambium tissue and sequenced two {DNA} barcodes (rbc{L}a and mat{K}) to aid in species identification. {W}e also collected fertile herbarium specimens from other species (trees/shrubs/herbs) inside and outside the plots. {T}he selected {DNA} barcodes were useful at the family level but failed to identify specimens at the species level. {B}ased on {DNA} barcoding identification, the most abundant families in the plots were {B}urseraceae, {F}abaceae, {M}eliaceae, {M}oraceae, {M}yristicaceae and {S}apotaceae. {O}ne third of the images of sampled plants posted on the i{N}aturalist website were identified by the community to species level. {N}ew approaches, including the sequencing of the {ITS} region and fast evolving {DNA} plastid regions, remain to be tested for their utility in the identification of specimens at lower taxonomic levels in floristic inventories in the {A}mazon region.}, keywords = {{DNA} barcoding ; {F}rench {G}uiana-{B}razil border ; mat{K} ; rbc{L}a ; tree inventory ; {T}umucumaque ; {GUYANE} {FRANCAISE} ; {BRESIL} ; {AMAZONIE} ; {TUMUCUMAQUE} {MONT}}, booktitle = {}, journal = {{A}cta {A}mazonica}, volume = {52}, numero = {1}, pages = {29--37}, ISSN = {0044-5967}, year = {2022}, DOI = {10.1590/1809-4392202101413}, URL = {https://www.documentation.ird.fr/hor/fdi:010084540}, }