@incollection{fdi:010084170,
  title = {{Z}ika virus targets multiple tissues and cell types during the first trimester of pregnancy},
  author = {{J}abrane-{F}errat, {N}. and {V}eas, {F}rancisco},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he 2016 {Z}ika virus ({ZIKV}) outbreak in the {A}mericas has been characterized by an increased association frequency of fetal neuropathological abnormalities. {T}o have a comprehensive and accurate knowledge of key elements of the clinically observed neurologic dysfunctions in {Z}ika-infected babies, {ZIKV} transmission from mother to fetus needs to be deeply studied. {T}hus, it is important to determine the role of both virus-targeted cells and tissues within the mother-fetus interface. {C}ellular tropism and mechanisms of {ZIKV} transmission from mother to the fetus during early pregnancy still remain unknown on many aspects. {T}o improve the characterization of the maternal-fetal {ZIKV} transmission, we have set up an ex vivo model using an organ culture approach with a light-invasive sampling from the first trimester of pregnancy samples. {T}hus, here we provide evidence that circulating epidemic {ZIKV} strains from {L}atin {A}merica widely target and destroy reproductive tissues, including the decidua basalis, fetal placenta, and umbilical cord. {I}n addition, we show that {ZIKV} is able to differentially replicate in a large range of both maternal and fetal cells, including decidual fibroblasts and macrophages, fetal trophoblast and {H}ofbauer cells, as well as umbilical cord mesenchymal stem cells. {T}his primary and broad {ZIKV} cellular tropism and the resulting abundant cytopathic-induced tissue effects during the first trimester of pregnancy show the upstream path of clinically observed congenital damages.},
  keywords = {},
  booktitle = {{Z}ika virus : methods and protocols},
  numero = {2142},
  pages = {235--249},
  address = {{N}ew {Y}ork},
  publisher = {{H}umana {P}ress},
  series = {{M}ethods in {M}olecular {B}iology},
  year = {2020},
  DOI = {10.1007/978-1-0716-0581-3_18},
  ISBN = {978-1-0716-0580-6},
  URL = {https://www.documentation.ird.fr/hor/fdi:010084170},
}