@article{fdi:010083952,
  title = {{A}lkaliphilus serpentinus sp. nov. and {A}lkaliphilus pronyensis sp. nov., two novel anaerobic alkaliphilic species isolated from the serpentinite-hosted {P}rony {B}ay {H}ydrothermal {F}ield ({N}ew {C}aledonia)},
  author = {{P}ostec, {A}. and {Q}u{\'e}m{\'e}neur, {M}arianne and {L}ecoeuvre, {A}. and {C}habert, {N}. and {J}oseph, {M}anon and {E}rauso, {G}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}wo novel anaerobic alkaliphilic strains, designated as {L}ac{TT} and {L}ac{VT}, were isolated from the {P}rony {B}ay {H}ydrothermal {F}ield ({PBHF}, {N}ew {C}aledonia). {C}ells were motile, {G}ram-positive, terminal endosporeforming rods, displaying a straight to curved morphology during the exponential phase. {S}trains {L}ac{TT} and {L}ac{VT} were mesophilic (optimum 30 degrees {C}), moderately alkaliphilic (optimum p{H} 8.2 and 8.7, respectively) and halotolerant (optimum 2% and 2.5% {N}a{C}l, respectively). {B}oth strains were able to ferment yeast extract, peptone and casamino acids, but only strain {L}ac{TT} could use sugars (glucose, maltose and sucrose). {B}oth strains disproportionated crotonate into acetate and butyrate. {P}hylogenetic analysis revealed that strains {L}ac{TT} and {L}ac{VT} shared 96.4% 16{S} r{RNA} gene sequence identity and were most closely related to {A}. peptidifermentans {Z}-7036, {A}. namsaraevii {X}-07-2 and {A}. hydrothermalis {F}at{MR}1 (95.7%-96.3%). {T}heir genome size was of 3.29 {M}b for strain {L}ac{TT} and 3.06 {M}b for strain {L}ac{VT} with a {G} + {C} content of 36.0 and 33.9 mol%, respectively. {T}he {ANI} value between both strains was 73.2 %. {F}inally, strains {L}ac{TT} (={DSM} 100337 = {JCM} 30643) and {L}ac{VT} (={DSM} 100017 = {JCM} 30644) are proposed as two novel species of the genus {A}lkaliphilus, order {C}lostridiales, phylum {F}irmicutes, {A}lkaliphilus serpentinus sp. nov. and {A}lkaliphilus pronyensis sp. nov., respectively. {T}he genomes of the three {A}lkaliphilus species isolated from {PBHF} were consistently detected in the {PBHF} chimney metagenomes, although at very low abundance, but not significantly in the metagenomes of other serpentinizing systems (marine or terrestrial) worldwide, suggesting they represent indigenous members of the {PBHF} microbial ecosystem.},
  keywords = {{F}irmicutes ; {A}lkaliphilus ; {A}naerobic ; {H}ydrothermal vents ; {S}erpentinization ; {N}ew {C}aledonia ; {NOUVELLE} {CALEDONIE} ; {PACIFIQUE} ; {PRONY} {BAIE}},
  booktitle = {},
  journal = {{S}ystematic and {A}pplied {M}icrobiology},
  volume = {44},
  numero = {2},
  pages = {126175 [9 ]},
  ISSN = {0723-2020},
  year = {2021},
  DOI = {10.1016/j.syapm.2020.126175},
  URL = {https://www.documentation.ird.fr/hor/fdi:010083952},
}