@article{fdi:010083793,
  title = {{T}he natural antisense transcript {DONE}40 derived from the lnc{RNA} {ENOD}40 locus interacts with {SET} domain protein {ASHR}3 during inception of symbiosis in {A}rachis hypogaea},
  author = {{G}anguly, {P}. and {R}oy, {D}. and {D}as, {T}. and {K}undu, {A}. and {C}artieaux, {F}abienne and {G}hosh, {Z}. and {D}asgupta, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he long noncoding {RNA} {ENOD}40 is required for cortical cell division during root nodule symbiosis ({RNS}) of legumes, though it is not essential for actinorhizal {RNS}. {O}ur objective was to understand whether {ENOD}40 was required for aeschynomenoid nodule formation in {A}rachis hypogaea. {A}h{ENOD}40 express from chromosome 5 (chr5) ({A}h{ENOD}40-1) and chr15 ({A}h{ENOD}40-2) during symbiosis, and {RNA} interference of these transcripts drastically affected nodulation, indicating the importance of {ENOD}40 in {A}. hypogaea. {F}urthermore, we demonstrated several distinct characteristics of {ENOD}40. (i) {N}atural antisense transcript ({NAT}) of {ENOD}40 was detected from the {A}h{ENOD}40-1 locus (designated as {NAT}-{A}h{DONE}40). (ii) {B}oth {A}h{ENOD}40-1 and {A}h{ENOD}40-2 had two exons, whereas {NAT}-{A}h{DONE}40 was monoexonic. {R}everse-transcription quantitative {PCR} analysis indicated both sense and antisense transcripts to be present in both cytoplasm and nucleus, and their expression increased with the progress of symbiosis. (iii) {RNA} pull-down from whole cell extracts of infected roots at 4 days postinfection indicated {NAT}-{A}h{DONE}40 to interact with the {SET} ({S}u(var)3-9, enhancer of {Z}este and {T}rithorax) domain containing absent small homeotic disc ({ASH}) family protein {A}h{ASHR}3 and this interaction was further validated using {RNA} immunoprecipitation and electrophoretic mobility shift assay. (iv) {C}hromatin immunoprecipitation assays indicate deposition of {ASHR}3specific histone marks {H}3{K}36me3 and {H}3{K}4me3 in both of the {ENOD}40 loci during the progress of symbiosis. {ASHR}3 is known for its role in optimizing cell proliferation and reprogramming. {B}ecause both {ASHR}3 and {ENOD}40 from legumes cluster away from those in actinorhizal plants and other nonlegumes in phylogenetic distance trees, we hypothesize that the interaction of {DONE}40 with {ASHR}3 could have evolved for adapting the nodule organogenesis program for legumes.},
  keywords = {{ENOD}40 ; {DONE}40 ; {ASHR}3 ; long noncoding {RNA} ; natural antisense transcript ; root nodule symbiosis},
  booktitle = {},
  journal = {{M}olecular {P}lant-{M}icrobe {I}nteractions},
  volume = {34},
  numero = {9},
  pages = {1057--1070},
  ISSN = {0894-0282},
  year = {2021},
  DOI = {10.1094/mpmi-12-20-0357-r},
  URL = {https://www.documentation.ird.fr/hor/fdi:010083793},
}